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Science and Islam

The Nutfa Hadith and Embryology: The 42-Day Miracle

86 min read 19167 words

The authentic hadith in Ṣaḥīḥ Muslim states that once forty-two nights pass over the nuṭfa (the early embryo), Allah sends an angel who forms it and creates its hearing, sight, skin, flesh, and bones, then determines whether it is male or female. This post compiles the embryological evidence that the two most testable claims in that hadith — the onset of bone ossification and the determination of the embryo’s sex — both occur at almost exactly day 42, the end of the sixth week and the start of the seventh.

About this work This is an English rendering of Ḥadīth al-Nuṭfa: A Scientific Miracle with Evidence, and a Discussion of the Hadith’s Chains of Transmission by (Dhū al-Qaʿda 1447 / October 2025). Every source, quotation, and reference in the original has been preserved.

The Hadith Under Discussion

The lead narration comes through Ḥudhayfa ibn Asīd al-Ghifārī (RA), recorded by Imām Muslim. It is one of the strongest formulations of the embryological hadith because it fixes the moment of formation at forty-two nights.

Ṣaḥīḥ Muslim 2645 — Ḥudhayfa ibn Asīd al-Ghifārī (RA) “إِذَا ‌مَرَّ ‌بِالنُّطْفَةِ ثِنْتَانِ وَأَرْبَعُونَ لَيْلَةً بَعَثَ اللهُ إِلَيْهَا مَلَكًا، فَصَوَّرَهَا، وَخَلَقَ سَمْعَهَا وَبَصَرَهَا وَجِلْدَهَا وَلَحْمَهَا وَعِظَامَهَا، ثُمَّ قَالَ: يَا رَبِّ أَذَكَرٌ أَمْ أُنْثَى؟ فَيَقْضِي رَبُّكَ مَا شَاءَ، وَيَكْتُبُ الْمَلَكُ، ثُمَّ يَقُولُ: يَا رَبِّ أَجَلُهُ؟ فَيَقُولُ رَبُّكَ مَا شَاءَ، وَيَكْتُبُ الْمَلَكُ، ثُمَّ يَقُولُ: يَا رَبِّ رِزْقُهُ؟ فَيَقْضِي رَبُّكَ مَا شَاءَ، وَيَكْتُبُ الْمَلَكُ، ثُمَّ يَخْرُجُ الْمَلَكُ بِالصَّحِيفَةِ فِي يَدِهِ، فَلَا يَزِيدُ عَلَى مَا أُمِرَ، وَلَا يَنْقُصُ”.

“When forty-two nights have passed over the nuṭfa, Allah sends an angel to it who forms it and creates its hearing, its sight, its skin, its flesh, and its bones. Then he says: ‘O Lord, male or female?’ and your Lord decrees what He wills and the angel records it. Then he says: ‘O Lord, his lifespan?’ and your Lord says what He wills and the angel records it. Then he says: ‘O Lord, his provision?’ and your Lord decrees what He wills and the angel records it. Then the angel departs with the sheet in his hand, neither adding to what he was commanded nor subtracting from it.”

Grade: Ṣaḥīḥ · Muslim (8/45)

The wording is reproduced from the printed text of Ṣaḥīḥ Muslim below.

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The hadith therefore states that hearing, sight, flesh, bone, and the embryo’s sex are formed at the close of the sixth week and the beginning of the seventh — at forty-two nights (six weeks) from fertilisation.

We will see roughly fifty references confirming that ossification begins in the embryo around day 42; roughly sixty references that the embryo’s sex is not chromosomally settled at 100% until day 42 (end of the sixth week, start of the seventh); and dozens more establishing the timing of gonadal sex (the determination of sex according to the genital organs). Flesh, the eye, and the ear are deliberately left out of the argument, so that no one can claim the Prophet ﷺ simply observed them with the naked eye in miscarried fetuses — the eye and ear are in fact visible to the naked eye in a fetus miscarried from the second month, unlike the embryo’s sex and the onset of bone ossification, which could not be known except under the electron microscope.

So that it is not said that Islam borrowed this from the Greeks, the author refers the reader to his research titled Greek Embryology Compared with the Noble Qur’an and the Sunnah (here).

If it is objected that the Prophet ﷺ mentioned the number by coincidence, we reply: a coincidence in the embryo’s sex — but is it also coincidence in the bones? And even if both of these examples were granted as coincidence, what about the rest of the examples that can be presented? An opponent has only a limited budget of “coincidence” to spend, but he cannot say “coincidence” every single time — if that is his argument, it collapses once ten further examples like these are produced.

After the scientific evidence, a dedicated ḥadīth-methodology section will follow on the chains (ṭuruq) of the nuṭfa hadith, demonstrating that the correct formulation is the one cited above, narrated in Ṣaḥīḥ Muslim from Ḥudhayfa ibn Asīd.

It has also been circulated that there is a consensus (ijmāʿ) among the Muslim scholars that the bones, flesh, hearing, sight, and sex of the embryo are formed on day 120 (three forties). This will be shown to be incorrect: most scholars hold that these organs and the sex of the embryo take shape at the start of the second forty (forty-something nights), and the consensus regarding day 120 is in fact the consensus that the ensoulment (nafkh al-rūḥ) occurs on day 120 from fertilisation.

Bone Formation Begins Around Day 42

All bones in the body originate from ossification centres,1 and the clavicle is the first bone to begin its formation (osteogenesis) through ossification in the embryo;2 we will see that this ossification takes place at the end of the sixth week and the start of the seventh. Ossification of the upper limbs likewise begins in the seventh week, as does that of the lower limbs, the vertebrae of the spinal column, the skull, the ribs, the scapula, the humerus, the femur, the tibia, the maxilla, and the palate. Most of the bones, as the sources below show, begin ossifying at the end of the sixth week.

Gray’s Anatomy states that the clavicle begins to ossify before any other bone in the body, in stages 18–20 — that is, 42 to 50 days post-fertilisation:3

Gray’s Anatomy “The clavicle begins to ossify before any other bone in the body. The shaft of the bone undergoes intramembranous ossification in two primary centres, medial and lateral, in stages 18–20 (42–50 postfertilization days).”

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In Pediatric Orthopedic Deformities, Dr. Frederic Shapiro places the onset of skeletal ossification in the seventh week, on day 44.4

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The study Development and Growth of the Normal Cranial Vault: An Embryologic Review presented a table of embryonic development in which ossification begins on day 43.5

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The reference article Embryology, Bone Ossification dates the onset of the process to between the sixth and seventh weeks:6

Embryology, Bone Ossification (NIH / StatPearls) “Bone ossification, or osteogenesis, is the process of bone formation. This process begins between the sixth and seventh weeks of embryonic development.”

The article Bone Formation and Development, published by Oregon State University, agrees:7

Oregon State University — Bone Formation and Development “By the sixth or seventh week of embryonic life, the actual process of bone development, ossification (osteogenesis), begins.”

An ossification table compiled by the embryologist Franklin P. Mall shows ossification beginning around day 39.8

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His table for ossification of the arm begins at day 39,9 as does his table for the skull.10

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The skull ossification table likewise opens at day 39.

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His table for the legs begins at day 42.11

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The study Resources for innovative learning of anatomy and foot ossification: Graphic design and virtual reality12 presented eight tables on the ossification of the foot bones; according to the eighth table, the first to ossify were the phalanges, in the seventh week.

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One of the most important embryology textbooks in the world, Larsen’s Human Embryology, notes that skeletal ossification begins on day 43:13

Larsen’s Human Embryology “Skeletal ossification begins.”

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The study The early development and ossification of the human clavicle — an embryologic study states plainly that the clavicle is the first bone to ossify in the developing embryo:14

Ogata & Uhthoff — The Early Development and Ossification of the Human Clavicle “The clavicle is the first bone to ossify in the developing embryo.”

The same study fixes the timing of that ossification to the sixth week:15

Ogata & Uhthoff — timing of clavicular ossification “Morphologic studies of the early development of the clavicle were carried out in 46 human embryos and fetuses ranging in age from 6 to 12 weeks. We confirmed that the clavicle is formed by two membranous primary ossification centers appearing by 6 weeks and fusing approximately 1 week later.”

It then presented a table of the stages of clavicular ossification beginning in the sixth week.16

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The medical journal American Journal of Diseases of Children presented tables for the ossification of all the embryo’s bones, appendicular and axial, and the first to ossify were the jaw and the clavicle in the seventh week.17

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The tables continue across the axial and appendicular skeleton.

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The series concludes with the remaining skeletal elements.

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The Thieme Atlas of Anatomy gives a table of embryonic bone ossification in which the first ossification begins in the middle of the second month (roughly six weeks) — for the clavicle, ulna, radius, tibia, fibula, and femur.

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A further table from the same atlas details the sequence.

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Pediatric Bone, Biology and Diseases states that nearly all primary ossification centres appear between weeks 7 and 12:18

Pediatric Bone, Biology and Diseases “Almost all primary ossification centers appear between weeks 7 and 12 of embryonic life in humans.”

In Imaging of the Sternocostoclavicular Region, Professor of Radiology at Aarhus University Dr. Anne Grethe Jurik writes that the clavicle’s two membranous primary centres appear in the sixth week:19

Anne Grethe Jurik — Imaging of the Sternocostoclavicular Region “The clavicle is the only long bone that develops almost entirely by a process of intramembranous ossification, with a direct transition from mesenchyme to ossified bone without the intermediate step of cartilage formation. In the embryo the clavicle is formed by two membranous primary centres appearing in the 6th week and fusing approximately 1 week later.”

Disorders of the Shoulder, Diagnosis & Management places the onset of fetal ossification in the clavicle at around eight weeks’ gestation:20

Disorders of the Shoulder, Diagnosis & Management “Fetal ossification begins in the clavicle at around 8 weeks’ gestation, followed by the mandible, vertebral bodies, and neural arches around 9 weeks.”

Here a recurring conversion must be noted: “8 weeks’ gestation” — counted from the last menstrual period — equals 6 weeks of embryonic age, counted from fertilisation.^22

Human Anatomy, A Complete Systematic Treatise (Sir Henry Morris, 1895) places the appearance of the primary clavicular nucleus in the sixth week of embryonic life:21

Henry Morris — Human Anatomy, A Complete Systematic Treatise “Ossification. The clavicle is ossified from two centres. The primary nucleus appears in the sixth week of embryonic life.”

The International Record of Medicine and General Practice Clinics dates the earliest ossification to the end of the sixth week:22

International Record of Medicine and General Practice Clinics “The earliest ossification begins toward the end of the sixth week of fetal life.”

Post Mortem Imaging of the Fetus & Child (2025) states that fetal skeletal ossification commences at 8 weeks’ gestation23 — again equivalent to 6 weeks of embryonic age:^26

Post Mortem Imaging of the Fetus & Child “Fetal skeletal ossification commences at 8 weeks’ gestation.”

Principles of Anatomy and Physiology (Tortora & Derrickson, 2020) places cartilage formation and ossification in the sixth week of embryonic development:24

Tortora & Derrickson — Principles of Anatomy and Physiology “The embryonic ‘skeleton’, initially composed of mesenchyme in the general shape of bones, is the site where cartilage formation and ossification occur during the sixth week of embryonic development. Bone formation follows one of two patterns.”

The Textbook of Craniofacial Growth states that almost all bones begin ossification around the eighth week of intrauterine life (IUL):25

Textbook of Craniofacial Growth “Almost all the bones start ossification around 8th week of IUL.”

The eighth week of intrauterine life — i.e. gestational age, counted from the last menstrual period — equals the sixth week of embryonic age, counted from fertilisation.^29

The Color Atlas of Human Fetal and Neonatal Histology (2019) gives an ossification timeline beginning in the seventh week.26

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The Sobotta Atlas of Anatomy (17th ed.) notes the clavicle as the exception that emerges from week 7 without a cartilaginous precursor:27

Sobotta Atlas of Anatomy “Up to week 12, ossification centres (nuclei) can be found in all bones of the upper limb, apart from the wrist. The ossification centres of the wrist emerge only after birth (postnatally) between the first and eighth year of life. An exception is the clavicle, which emerges from week 7 without a cartilaginous precursor and thus derives directly from the mesenchyme (desmal ossification).”

Charles Sedgwick Minot’s Human Embryology records the clavicle as the first bone formed in the human embryo, ossifying during the seventh week:28

Charles Sedgwick Minot — Human Embryology “Clavicle… It is, as discovered by C. Bruch, the first bone formed in the human embryo, its ossification going on during the seventh week.”

The journal Colorado Medicine (1911) describes the clavicle as the first bone in the body to show signs of ossification, appearing around the sixth week:29

Colorado Medicine (1911) “The shaft of the clavicle, the first bone in the body to show signs of ossification, has usually been regarded as arising in membrane from a single center appearing at about the sixth week.”

Upper extremity, back of neck, shoulder, trunk, cranium, scalp, face (John Blair Deaver) repeats the same point, dating the shaft’s ossification to about the sixth week of fetal life:30

John Blair Deaver — Upper Extremity, Back of Neck, Shoulder… “The clavicle has two centers of ossification — one for the shaft, and one for the sternal end. Ossification in the shaft begins about the sixth week of fetal life. The clavicle is the first bone in the body to show signs of ossification.”

A Manual of Human Physiology (Leonard Landois) names the clavicle as the first bone to ossify, in the seventh week:31

Leonard Landois — A Manual of Human Physiology “The clavicle, according to Bruch, is not a membrane bone, but is formed in cartilage like the furculum of birds (Gegenbaur). At the 2nd month it is four times as large as the upper limb; it is the first bone to ossify at the 7th week.”

Dr. Mark Hill’s embryology navigator records the clavicle as the very first bone to ossify, in the sixth week, with the remaining upper-limb bones — humerus, ulna, and radius — beginning in late week six and early week seven.32

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The same source’s table continues for the rest of the upper limb.

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For the lower limb, the femur ossifies on day 43 and the tibia on day 43.33

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And the jaw ossifies in the sixth week.34

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The ASSH Manual of Hand Surgery describes ossified bone becoming apparent by the seventh week:35

ASSH Manual of Hand Surgery “Early mesenchymal condensation begins during the fifth week… By the seventh week, ossified bone becomes apparent, initially in primary ossification centers in the midpoints of long bones.”

The Textbook of General Anatomy with Systemic Anatomy, Radiological Anatomy, Medical Genetics (V. Subhadra Devi) records the first ossification — in the clavicle and skull — at around the fifth or sixth week.36

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Henry Gray’s Anatomy of the Human Body lists the seventh-week appearance of ossification points for the ribs, scapula, humerus, femur, tibia, intermaxillary bone, and palate:37

Henry Gray — Anatomy of the Human Body “Seventh week. The muscles begin to be perceptible. Points of ossification of the ribs, scapula, shafts of humerus, femur, tibia, intermaxillary bone, palate.”

Franz Keibel’s Manual of Human Embryology records the upper-limb bones — clavicle, humerus, ulna, radius — as the first to ossify, beginning in the sixth week for the clavicle and the seventh for the rest.38

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Another source confirms that the radius and ulna begin ossifying in the seventh week:39

Wheeless’ Textbook of Orthopaedics “Primary ossification centers at 8 weeks gestation in both radius and ulna.”

Essentials of Anatomy for Dentistry Students (D. R. Singh) shows upper-limb ossification beginning in the seventh week.40

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The ASSH Manual of Hand Surgery dates the appearance of the vertebral ossification centres to eight weeks’ gestation:41

ASSH Manual of Hand Surgery — vertebral ossification “The vertebral column ossifies in hyaline cartilage. There are three primary ossification centres for a typical vertebra; one in the centrum and one for each half of the neural arch. These appear at 8 weeks gestation.”

Eight weeks’ gestation again equals six weeks of embryonic age from fertilisation.^46

The Textbook of Clinical Embryology (Vishram Singh, 2020) notes that most limb ossification centres appear between the seventh and twelfth weeks:42

Vishram Singh — Textbook of Clinical Embryology “The primary centers of ossification of limb bones appear at different times in different bones, but most of them appear between 7th and 12th week, i.e., virtually all primary centers of ossification are present at birth and most of the secondary centers appear after birth.”

Ronald Dudek’s Embryology places the primary ossification centres of the clavicle, humerus, radius, and ulna at weeks 7–9, again naming the clavicle as the first bone in the body to ossify:43

Ronald Dudek — Embryology “At weeks 7–9, the primary ossification centers are seen in the clavicle, humerus, radius, and ulnar bones. The clavicle is the first bone in the entire body to ossify.”

The Elements of Anatomy gives a list of the first appearances of ossification: the clavicle first, in the sixth week, then the scapula, humerus, ulna, and tibia in the seventh.44

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The list continues for the remaining bones.

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Developmental Anatomy and Physiology of Children (Carol A. Chamley, 2005) names the clavicle as the first bone in the appendicular skeleton to ossify, in the sixth week:45

Carol A. Chamley — Developmental Anatomy and Physiology of Children “The clavicle develops initially by a process of intramembranous ossification, later forming growth cartilages at both ends of the developing bone. Furthermore, the clavicle is the first bone in the appendicular skeleton to ossify during week 6 of embryological development. Ossification begins in the long bones by week 8 of gestation.”

Anatomy and Human Movement (Palastanga & Soames) places the appearance of the primary shaft centre in the eighth week in utero46 — equal to the sixth week of embryonic age:

Palastanga & Soames — Anatomy and Human Movement “Ossification: A primary ossification centre appears in the shaft during the eighth week in utero.”

Cunningham’s Manual of Practical Anatomy dates the primary centres in the long bones to about eight weeks of intrauterine life:47

Cunningham’s Manual of Practical Anatomy “The ossification centres in the bodies of the long bones (primary ossification centres) appear at approximately 8 weeks of intrauterine life.”

Eight weeks of intrauterine life — gestational age from the last menstrual period — equals six weeks of embryonic age from fertilisation.

A table from Growth, Maturation, and Physical Activity closes the main catalogue.

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Other Sources

A range of further educational and clinical sources state the same conclusion in plainer terms. The Cleveland Clinic places the replacement of soft cartilage by bone — and the start of genital formation — in the seventh week:^53

Cleveland Clinic “Week 7: Bones begin replacing soft cartilage and genitals begin to form.”

BabyCenter notes the outline of the entire skeleton is established by seven weeks:^54

BabyCenter “7 weeks: Bone outlines for entire skeleton established.”

Lumen Learning dates the start of ossification to the sixth or seventh week of embryonic life:^55

Lumen Learning “By the sixth or seventh week of embryonic life, the actual process of bone development, ossification (osteogenesis), begins.”

Creative Diagnostics places it after seven weeks of embryonic development,^56 and OpenStax repeats the sixth-to-seventh-week window while detailing the two osteogenic pathways:^57

OpenStax — Anatomy and Physiology “By the sixth or seventh week of embryonic life, the actual process of bone development, ossification (osteogenesis), begins. There are two osteogenic pathways — intramembranous ossification and endochondral ossification — but bone is the same regardless of the pathway that produces it.”

CUNY OpenEd places it at approximately six weeks after fertilisation,^58 as does Bartleby (“about six weeks after fertilization”),^59 and the BC Open Textbook collection, which adds that ossification is distinct from calcification:^60

BC Open Textbook — Biology “Ossification, or osteogenesis, is the process of bone formation by osteoblasts… Ossification begins approximately six weeks after fertilization in an embryo.”

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This matches the noble hadith precisely, which places the formation of the bones at day 42:

Ṣaḥīḥ Muslim 2645 — the relevant clause “إِذَا ‌مَرَّ ‌بِالنُّطْفَةِ ثِنْتَانِ وَأَرْبَعُونَ لَيْلَةً بَعَثَ اللهُ إِلَيْهَا مَلَكًا، فَصَوَّرَهَا، وَخَلَقَ سَمْعَهَا وَبَصَرَهَا وَجِلْدَهَا وَلَحْمَهَا وَعِظَامَهَا…”

“When forty-two nights have passed over the nuṭfa, Allah sends an angel to it who forms it and creates its hearing, its sight, its skin, its flesh, and its bones…”

Grade: Ṣaḥīḥ · Muslim (8/45)

The Sex of the Fetus

Sex determination in vertebrates is not, as was first believed, the result of a simple hierarchical cascade of gene actions; it is rather the product of a complex network of positive and negative regulatory interactions.48

The notion that a Y sperm fertilising an X egg yields a male, and an X sperm fertilising an X egg yields a female, is an old idea more than a hundred years old. We have recently discovered that there are numerous genes on the X and Y chromosomes that act as the overseers of the chromosome’s work in forming the embryo’s sex. Among them are six genes whose malfunction causes sex reversal — so that the embryo emerges female despite being chromosomally XY, or male despite being chromosomally XX. These six genes operate only at the end of the sixth week and the start of the seventh, the most important of them being SRY and RSPO1. This means it is not enough for a Y sperm to fertilise an X egg for the embryo to become 100% male; the chromosomal pattern must continue correctly to the end of the sixth week (42 nights), which is decided only by the operation of these six genes. The start of the seventh week is the moment when the possibility of sex reversal closes.

We then found that the gonadal sex of the embryo is not determined until day 42 — that is, the embryo remains bipotential, capable of becoming either male or female, until day 42; only at day 42 do the germ cells begin to differentiate and divide into either male or female.

It is thus established that both chromosomal sex and gonadal sex are not determined until the end of the sixth week and the start of the seventh.

Chromosomal Sex

A note on the dating used by the sources: we will sometimes read “gestational age” (counted from the last menstrual period, i.e. roughly post-conception), which is two weeks longer than the “embryonic age” (counted from fertilisation). So the eighth week of gestation equals the sixth week from fertilisation, and the eighth week from the last menstrual period equals the sixth week of embryonic age.49

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A Y sperm fertilising an X egg does not always make the embryo male, nor is XX always female: there are six genes, operating only at the end of the sixth week, capable of reversing the embryo’s sex from male to female and from female to male. If this window passes safely (end of the sixth week, start of the seventh), the embryo’s sex will not reverse. It is therefore not enough for a Y sperm to fertilise an X egg for the embryo to become male; the chromosomal pattern must remain sound until the end of the sixth week — something that will occur only if these six genes operate at the end of the sixth week. Day 42 (end of the sixth, start of the seventh) thus has a pivotal role in determining the embryo’s sex, for it is the period in which the phenomenon of sex reversal closes 100%.

Sex Reversal

Sex reversal The differentiation of a gonadal or sexual phenotype that does not align with the genetic sex — for example, the formation of an ovary and a female phenotype in an XY animal, or testes in an XX animal.

There are seven genes that govern the embryo’s sex, a malfunction in any of which leads to sex reversal:50 SRY, SOX9, SOX8, FGF9, DAX1, RSPO1, and WNT4. All of them, however, are governed by SRY in the male and RSPO1 in the female, as a study on the genetics of disorders of sexual development explains:51

Genetics of Disorders of Sexual Development (Monash) “Gonadal differentiation is the first step of mammalian sex determination. In mammals, male sex determination is governed by SRY-dependent activation of SOX9, whereas female development involves RSPO1, an activator of the WNT/beta-catenin signaling pathway. Hormones such as androgens and anti-Müllerian hormone also affect sexual differentiation and are important for sex determination. This review will focus on the male pathway.”

SRY itself, for instance, was discovered when the DNA of four XX males was analysed: all were positive for Y-specific markers in the 35-kilobase region immediately adjacent to the pseudoautosomal boundary. That region was searched for conserved Y-linked sequences, an open reading frame was found, and the corresponding gene was named SRY (sex-determining region Y).52

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The reference BGDB Sexual Differentiation — Sex Determination presents the same genetic framework.53

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So too does the study Sex Determination and Gonadal Development in Mammals, which tabulates the genes implicated in sexual development.54

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The book The Genetics and Biology of Sex Determination distinguishes the male-promoting genes from those that oppose them:55

The Genetics and Biology of Sex Determination “While some genes such as SRY and SOX9 have been shown to influence sex determination towards maleness, others such as DAX1 and WNT4 have been shown to prevent it, or even to possibly influence ovarian formation.”

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The work Anomalies of Human Sexual Development: Clinical Aspects and Genetic Analysis spells out how disorders of these genes produce sexual ambiguity and full sex reversal, and that the system is dosage-sensitive:56

Eric Vilain — Anomalies of Human Sexual Development “Disorders of human sex determination result in malformations of the external and internal genitalia. These malformations may vary from sexual ambiguity to complete sex reversal (XY female, XX male)… Until recently, only transcription factors such as SRY, SOX9, DAX1, WT1 and SF1 were known to be responsible for abnormal gonadal development and sexual ambiguity. Gonadal dysgenesis may be isolated, as in the case of SRY mutations, or associated with abnormal development of other organs, such as bone or adrenals… WNT4 is a new sex-determining molecule. Deletions of Wnt4 were shown to be responsible for the masculinization of XX mouse pups, while its duplication and overexpression in humans leads to XY sex reversal. Similarly, duplications of loci containing DAX1 or SOX9 have also been shown to cause sex reversal. These results support the emerging concept that mammalian sex determination is dosage sensitive at multiple steps of its pathway.”

The dedicated study Sex Reversal frames the process as three phases, during the first two of which the gonad remains plastic and its fate can still be switched:57

Weber & Capel — Sex Reversal “We can think of sex determination happening in three distinct phases: an initiation phase (when the molecular pathway triggering differentiation of the ovary or the testis is activated), a maintenance phase (whereby the ovary or testis pathway is perpetuated and reinforced while the opposite pathway is repressed), and a later, stabilization phase (when the ovaries or testes become functional). During the initiation and maintenance phases, the gonad is still plastic and sexual fate can be switched to the opposing pathway. Sex reversal often occurs because of a failure to maintain the initiated pathway or a failure to repress the opposite pathway.”

Sertoli Cell Biology confirms experimentally that introducing Sry into an XX gonad produces a testis:58

Sertoli Cell Biology “Expression of Sry in an XX gonad leads to development of a testis, whereas mouse XY embryos carrying a Y chromosome deleted for Sry develop ovaries.”

The timing of this divergence is fixed at the end of the sixth week, as Exploring the Dirty Side of Women’s Health states:59

Exploring the Dirty Side of Women’s Health “At the end of the sixth week, male and female genital systems are indistinguishable in appearance… The ambisexual or indifferent phase of genital development ends at this point, and from the seventh week on the male and female systems pursue diverging pathways… Maleness is actively induced by a sex-determining transcription factor encoded on the sex-determining region of the Y chromosome (SRY). If the factor is absent or defective, female development occurs.”

Effective Management of Bladder and Bowel Problems in Children describes the same switch — SRY present yields testes, SRY absent yields ovaries:60

Effective Management of Bladder and Bowel Problems in Children “Under the influence of the SRY gene on the Y chromosome, the primitive sex cords develop to form the testis… In the absence of the SRY gene, the gonadal cortical layer containing the germ cells further develops, while regression occurs in the medullary layer, resulting in the formation of ovaries.”

The study SRY — Sex Reversal sets out the gene’s history and its decisive role:61

Stephen S. Wachtel — SRY — Sex Reversal “The SRY (sex-determining region Y) gene was discovered in 1990 by Andrew Sinclair and colleagues… SRY is the switch that determines the sex of the mammalian embryo. In the presence of a functioning SRY gene, the indifferent embryonic gonad becomes a testis… In the absence of the SRY gene, the gonad becomes an ovary… But errors in the expression and regulation of SRY may lead to development of testes in XX embryos and failure of testicular development in XY embryos.”

A clinical case study, XY Sex Reversal Associated with a Nonsense Mutation in SRY, documents an XY individual who developed as female because of a defective SRY:62

McElreavey et al. — XY Sex Reversal Associated with a Nonsense Mutation in SRY “Sex determination in humans is mediated through the expression of a testis-determining gene on the Y chromosome… termed SRY. Here we describe an XY sex-reversed female with pure gonadal dysgenesis who harbors a de novo nonsense mutation in the SRY open reading frame (SRY-orf).”

Finally, The Genetics and Biology of Sex Determination summarises how the dosage of these very genes can reverse sex in either direction:63

The Genetics and Biology of Sex Determination “Duplication of chromosomal regions containing sex-determining genes lead to XY sex reversal (DAX1 and WNT4) or XX sex reversal (SOX9).”

The SRY Gene

Role of SRY

SRY is the testis-determining factor (TDF) — the master switch for the sexual differentiation of the gonad:64

Sex Chromosomes and Sex Determination in Vertebrates “The plausible assumption that SRY is TDF, that is, the master switch for sexual differentiation of the gonad.”

When SRY is expressed in the bipotential gonad during a critical developmental window, it activates SOX9 and the whole male-promoting network, producing a testis; otherwise the female-promoting network prevails and an ovary forms:65

Early Gonadal Development and Sex Determination in Mammals “The SRY on the Y chromosome is the ‘master switch’ for testis determination in mammals. When SRY is expressed in bipotential gonads during a critical window of fetal development, SOX9 expression and a male-promoting regulatory network are activated, resulting in testis differentiation. In contrast, ovary differentiation will be triggered when the balance is tilted towards a female-promoting regulatory network.”

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A paper carrying that very description in its title — Sry: the master switch in mammalian sex determination — makes the same point.66

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An ontogenic and morphological study of gonadal formation in genetically modified, sex-reversed XYPOS mice frames the whole question in terms of SRY’s presence or absence:67

Umemura et al. — gonadal formation in XYPOS mice “Mammalian sexual fate is determined by the presence or absence of sex-determining region of the Y chromosome (Sry) in the ‘bipotential’ gonads.”

A gene-editing study in pigs confirms that disrupting SRY drives male-to-female reversal:68

Kurtz et al. — Knockout of the HMG domain of the porcine SRY gene “The sex-determining region on the Y chromosome (SRY) is thought to be the central genetic element of male sex development in mammals. Pathogenic modifications within the SRY gene are associated with a male-to-female sex reversal syndrome in humans and other mammalian species, including rabbits and mice.”

Maternal, Fetal, & Neonatal Physiology names SRY and Wnt4 as the key genes controlling the gonadal ridge’s fate:69

Susan Blackburn — Maternal, Fetal, & Neonatal Physiology “Genetic control of sex determination involves multiple genes and factors in male and female embryos. Key genes controlling development of the gonadal ridge into the bipotential gonad include SRY (sex region on the Y chromosome) and Wnt4.”

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When SRY Activates

This is the decisive question, and the sources converge with striking consistency: SRY switches on in the human embryo at about day 42. The reference Hormones states it directly:70

Norman & Henry — Hormones “The SRY gene product, SRY, is detected in the bipotential gonad of XY individuals at about 42 days.”

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The Hidden Relay fixes both the onset and the decline of human SRY expression:71

Peter de Boer — The Hidden Relay “In mice, it is only expressed over a relatively short period… The expression begins at 10.5 days, peaks between 11 and 11.5 days and then vanishes again. In humans, it appears 42 days after fertilization and decreases from day 53. SRY is a transcription factor of a specific class.”

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Principles for Evaluating Health Risks in Children gives the same window with a peak at day 44:72

Principles for Evaluating Health Risks in Children “SRY gene expression starts at 41–44 days after ovulation, peaks at day 44, and continues at low levels thereafter (Hanley et al., 2000).”

Obstetrics & Gynaecology: An Evidence-based Text for MRCOG places activation at the end of the sixth week:73

Obstetrics & Gynaecology (MRCOG) “In an XY fetus, activation of the SRY (sex-determining region of the Y chromosome) gene at the end of week 6 guides the indifferent gonad to commence development into a testis.”

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The study The molecular pathways underlying early gonadal development gives the figure of 41–42 days:74

Yang, Workman & Wilson — The molecular pathways underlying early gonadal development “Gonadal sex determination occurs around 41–42 days in human embryos, signified by the upregulation of SRY gene expression in XY embryos (Hanley et al. 2000, Del Valle et al. 2017, Mamsen et al. 2017).”

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The same Principles for Evaluating Health Risks in Children notes that steroidogenic-cell expression follows testis determination from around day 42 onward:75

Principles for Evaluating Health Risks in Children “Its expression has been demonstrated in the bipotential gonad from 32 days post conception and, following testis determination (around 42 days onward), its expression is…”

The textbook In-Vitro Fertilization dates the identification of the primordial gonads to 37–42 days after fertilisation:76

Elder & Dale — In-Vitro Fertilization “Primordial gonads can be identified on either side of the central dorsal aorta between 37 and 42 days after fertilization (gestational age 8–9 weeks) as a medial thickening of the mesodermal epithelium that lines the coelom.”

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Sertoli Cell Biology gives the human window as 41–44 days:77

Sertoli Cell Biology “The task of diverting gonad development toward the male pathway is accomplished in mammals by the expression of Sry/SRY, which is activated in a subset of somatic cells of the XY gonad at E10.5 in mice and between 41 and 44 days of development in humans.”

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The Color Atlas of Human Fetal and Neonatal Histology places the first step of testicular differentiation toward the end of the sixth post-fertilisation week:78

Color Atlas of Human Fetal and Neonatal Histology “The first step in testicular differentiation, the transformation of the bipotential gonad into the testis, begins toward the end of the sixth postfertilization week. In the presence of sex-determining region Y (SRY) protein, also known as testis-determining factor, the primitive gonadal cords of the bipotential gonad transform into solid testicular seminiferous cords.”

Basics of Gynecology for Examinees presents the same timing in tabular form.79

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So too does Comprehensive Gynecology.80

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The Nature paper Human sex reversal is caused by duplication or deletion of core enhancers upstream of SOX9 states the human onset at six weeks post-ovulation:81

Croft et al. (Nature) — Human sex reversal… upstream of SOX9 “In most mammals including humans, testis determination is initiated by the Y-linked gene SRY. Sry is expressed transiently from 10.5 days post coitum (dpc) in mouse embryos, while in humans expression begins at 6 weeks post ovulation and is maintained throughout gestation.”

Essentials of Human Embryology likewise places the start at the end of the sixth week:82

Rosemol Xaviour — Essentials of Human Embryology “The sexual differentiation of genetic males commences at the end of the sixth week by the expression of SRY gene on Y chromosome.”

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Human Reproductive and Prenatal Genetics describes the divergence of the gonads at about eight weeks’ gestation, with SRY triggering AMH synthesis between weeks eight and nine:83

Qiao & Leung — Human Reproductive and Prenatal Genetics “At about 8 weeks gestation… the gonads in males will have begun to form visible testicular cords, spermatogenic veins, and hormone-secreting Sertoli and Leydig cells… Between the eighth and ninth weeks of gestation, the sex-determining region on the Y chromosome, SRY, gene… triggers the synthesis and secretion of AMH… from Sertoli cells. During the ninth week, the interstitial Leydig cells will begin secreting testosterone.”

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Here again the eighth week of gestation equals the sixth week of embryonic age from fertilisation, and the ninth equals the seventh.

The Encyclopedia of Reproduction dates the formation of the testis to week 7 through the expression of SRY together with SOX9, GATA4, and DAX1:84

Encyclopedia of Reproduction “Formation of the testis begins at week 7 and results from expression of the SRY gene on the Y chromosome and the genes SOX9, GATA4, and DAX1.”

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SRY’s Effect on the Female Fetus

SRY can be transferred by error during meiosis onto the X chromosome, producing XY females and XX males. Such a female carries an X bearing the SRY gene; if her X is then fertilised by an X sperm, the embryo emerges male despite being XX. The literature documents both directions of this anomaly.

Genes and Mechanisms in Vertebrate Sex Determination “The majority of human XX males possess Y-derived DNA sequences, transferred to the paternal X chromosome by aberrant X–Y interchange during meiosis.”

The mechanism is described identically in Sex Determination and Gonadal Development in Mammals:85

Wilhelm, Palmer & Koopman — Sex Determination and Gonadal Development in Mammals “XX sex reversal often occurs in humans through the transfer of TDY onto the X chromosome due to an illegitimate recombination between the X and Y in male meiosis.”

There is, conversely, a documented case of complete masculinisation without SRY at all — a study titled SRY-negative 46,XX male with normal genitals, complete masculinization and infertility86 — showing that the network, not the single gene, ultimately governs the outcome.

The WNT4 Gene

Role and Sex Reversal

The same Maternal, Fetal, & Neonatal Physiology passage that lists the key sex-determining genes names Wnt4 alongside SRY:87

Susan Blackburn — Maternal, Fetal, & Neonatal Physiology “Key genes controlling development of the gonadal ridge into the bipotential gonad include SRY (sex region on the Y chromosome) and Wnt4.”

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WNT4 works in the opposite direction to SRY — it promotes the female pathway, so disrupting it can masculinise a female and overexpressing it can feminise a male:88

The Genetics and Biology of Sex Determination “Targeted deletion of Wnt4 results in the masculinization of XX mice. We have recently shown that overexpression of WNT4 in humans results in XY sex reversal, as observed with overexpression of DAX1.”

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The study Sex Reversal explains how the balance tips in either direction, with Wnt4 as the female-side counterweight to Sox9:89

Weber & Capel — Sex Reversal “Sex determination operates like a sensitive balance where any dominating factor may throw the balance in favor of one outcome over the other… Sex reversal can also go the other way: when Wnt4, which activates other female pathway genes and represses Sox9, is mis-regulated or missing, the male pathway can dominate and lead to the development of a testis and a male phenotype in an XX individual (even in the absence of the Sry gene).”

The same dosage sensitivity is summarised by the Genetics of Disorders of Sexual Development:90

Genetics of Disorders of Sexual Development (Monash) “WNT4 mutation also contributes towards sex reversal. It was known that lack of WNT4 activity promotes masculinisation in female, while excess WNT4 activity promotes feminization of male.”

When WNT4 Activates

Yen & Jaffe’s Reproductive Endocrinology sets out the timing of the WNT/β-catenin pathway in tabular form.91

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In this source the abbreviation “wpc” means weeks post-conception; the eighth week post-conception equals the sixth week of embryonic age from fertilisation. A study on the same signalling pathway dates its onset to the seventh week:92

Ma et al. — Breast Milk Composition and Infant Metabolism “The Wnt/β-catenin signaling cascade is critical for lung formation during early stages. Wnt signaling occurs at 7 weeks of pregnancy, peaks at 17 weeks, and subsequently declines at 21 weeks.”

The DAX1 Gene

Genes and Mechanisms in Vertebrate Sex Determination identifies DAX1 as an X-linked, ovary-determining gene whose duplication reverses male to female:93

Genes and Mechanisms in Vertebrate Sex Determination “DAX1 maps to a region of the human X chromosome which, when duplicated, causes male-to-female sex reversal, and it has been suggested that Dax1 is an ovary-determining gene… Transgenic mice overexpressing Dax1 have been shown to undergo male-to-female sex reversal.”

A clinical reproductive text records the same association with adrenal hypoplasia and XY sex reversal:94

Falcone & Hurd — Clinical Reproductive Medicine and Surgery “Alterations in DAX1 expression are associated with sex reversal and adrenal hypoplasia congenita… Duplications of this gene cause sex-reversed XY females.”

The Encyclopedia of Reproduction lists DAX1 among the genes whose expression at week 7 forms the testis:95

Encyclopedia of Reproduction “Formation of the testis begins at week 7 and results from expression of the SRY gene on the Y chromosome and the genes SOX9, GATA4, and DAX1.”

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The WT1 Gene

Genes and Mechanisms in Vertebrate Sex Determination shows that suppressing WT1 impairs genitourinary development, the most severe defects causing gonadal dysgenesis and sex reversal:96

Genes and Mechanisms in Vertebrate Sex Determination “It is probable that the degree of inhibition of WT1 action correlates with the impairment of genitourinary development, with the most severe mutations leading to early gonadal dysgenesis and sex reversal of external and internal genitalia.”

The SOX9 Gene

SOX9 is downstream of SRY but powerful enough on its own to build a testis: introducing it into an XX mouse, or simply increasing its dosage, produces sex reversal toward maleness.97

The Genetics and Biology of Sex Determination “Sox9 is sufficient to induce testis formation in XX mice.”

The duplication effect is documented directly:98

Falcone & Hurd — Clinical Reproductive Medicine and Surgery “Increased dosages of SOX9 from duplication have been shown to cause autosomal sex reversal in XX individuals.”

When SOX9 Activates

The Encyclopedia of Reproduction again dates testis formation — driven by SRY together with SOX9 — to week 7:99

Encyclopedia of Reproduction “Formation of the testis begins at week 7 and results from expression of the SRY gene on the Y chromosome and the genes SOX9, GATA4, and DAX1.”

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Human Reproductive and Prenatal Genetics shows SOX9 driving AMH secretion between the eighth and ninth gestational weeks:100

Qiao & Leung — Human Reproductive and Prenatal Genetics “During the ninth week, the interstitial Leydig cells will begin secreting testosterone. AMH production by the Sertoli cell is activated by SOX9, a transcription factor from the SRY-related HMG box gene group, and inhibits the development of the Müllerian ducts into the fallopian tubes, uterus, cervix, and the interior part of the vagina.”

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Here again the eighth week of gestation equals the sixth week from fertilisation, and the ninth equals the seventh. Once SRY switches on, it immediately drives SOX9:101

Qiao & Leung — Human Reproductive and Prenatal Genetics “SRY expression is independent of the presence of germ cells. SRY increases the expression of the SRY-related HMG box-containing-9 (SOX9) gene.”

Genetics of Male Infertility describes SOX9 as both necessary and sufficient for testis determination:102

Genetics of Male Infertility “Once SRY is activated, Sertoli cells differentiate from supporting lineage by expressing SOX9. SOX9 (SRY-box 9) is a HMG box protein necessary and sufficient for testis determination.”

Pediatric Endocrinology repeats that SRY raises SOX9 expression.103

The DMRT1 Gene

The Genetics and Biology of Sex Determination lists the chromosomal region 9p24 — deleted in some XY females — which carries the transcription factors DMRT1 and DMRT2:104

The Genetics and Biology of Sex Determination “They include 9p24, a region deleted in some XY females that contains the transcription factors DMRT1 and DMRT2.”

When DMRT1 Activates

Pediatric Endocrinology places the sexually dimorphic expression of DMRT1 in human fetuses of six and seven weeks:105

Mark A. Sperling — Pediatric Endocrinology “Sexually dimorphic expression of Doublesexand MAB3-Related Transcription Factor 1 (DMRT1) was found in 6and 7-week-old human fetuses.”

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The RSPO1 Gene

When RSPO1 Activates

By the seventh week from fertilisation (the ninth gestational week), RSPO1 — the master female-side gene — has reached its peak. Pediatric Endocrinology records its rise in the developing ovary in the absence of SRY:106

Mark A. Sperling — Pediatric Endocrinology “In the absence of SRY, between 6 to 9 weeks of gestation, R-spondin-1 (RSPO1) expression increases in the developing human ovary. RSPO1 is a secreted factor.”

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Yen & Jaffe’s Reproductive Endocrinology tabulates the same timing.107

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Eight weeks post-conception (i.e. from the last menstrual period) equals six weeks from fertilisation.

Gonadal Sex

The studies are unanimous that, in terms of its genitalia, the embryo is neither male nor female until day 42: it remains bipotential — capable of becoming either — and the male embryo cannot be distinguished from the female by its genitalia until day 42. This is what scientists call the embryo’s gonadal sex. The germ cells begin to form early, but they do not begin differentiating into male or female genital organs until day 42.

Dr. Albert Singer, a pioneer of colposcopy, dates the indifferent gonad to about day 42 (Carnegie stage 17):108

Albert Singer — The Cervix “Until approximately 42 days, Carnegie stage 17, this ridge forms the indifferent gonad, and male and female embryos are morphologically indistinguishable.”

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The Textbook for MRCOG-1 fixes the same point — the primitive gonad looks identical in both sexes until day 42:109

Richa Saxena — Textbook for MRCOG-1 “The appearance of the primitive gonad is similar in both sexes until 42 days after fertilisation, when differentiation of seminiferous tubules occurs.”

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Fetal and Neonatal Physiology stresses that although the sex chromosomes are set at fertilisation, gonadal differentiation cannot be detected until about day 42:110

Polin, Fox & Abman — Fetal and Neonatal Physiology “Although the sex chromosome complement, and therewith the sex-determining genes, are established at fertilization, it is not until about 42 days that differentiation of the indifferent gonads into testes in the male or, slightly later, their development into ovaries in the female, can be detected.”

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A multiple-choice text in obstetrics and gynaecology gives the 12-mm (42-day) embryo as the cutoff:111

Hurry & Charles — Obstetrics & Gynecology “Until the 12-mm stage (42-day embryo) the fetal gonads are undifferentiated.”

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A Comprehensive Textbook of Obstetrics and Gynecology says the same plainly:112

Sadhana Gupta — A Comprehensive Textbook of Obstetrics and Gynecology “Male and female embryos are morphologically indistinguishable till 42 days.”

Essential Endocrinology places the start of differentiation around the fortieth day:113

C. R. Kannan — Essential Endocrinology “The primitive gonads, in embryos of both sexes, are bipotential. Around the 40th day, the bipotential gonad starts differentiating into a testis or an ovary.”

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The MRCOG evidence-based text repeats that the indifferent gonad begins testis development at the end of week 6:114

Obstetrics & Gynaecology (MRCOG) “In an XY fetus, activation of the SRY gene at the end of week 6 guides the indifferent gonad to commence development into a testis.”

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Dr. Rodolfo Rey’s Sexual Differentiation states that no sexual difference is visible until the sixth week:115

Rodolfo Rey et al. — Sexual Differentiation “No sexual difference can be observed in the gonads until the 6th week of embryonic life in humans.”

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The same source adds that six weeks elapse before the first signs appear:116

Rodolfo Rey et al. — Sexual Differentiation “6 weeks elapse in humans before the first signs of sex differentiation are noticed.”

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The study Early Gonadal Development and Sex Determination in Mammals puts the first appearance of testis cords at 6–7 weeks:117

Xie et al. — Early Gonadal Development and Sex Determination in Mammals “At approximately 6–7 weeks of pregnancy in humans (E12.5 in mice), testis cords are observed in XY gonads, indicating the start of sex differentiation in bipotential gonads.”

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The molecular pathways underlying early gonadal development gives both the 6–7-week window and the 41–42-day figure:118

Yang, Workman & Wilson — The molecular pathways underlying early gonadal development “In humans… the first signs of sex differentiation are observed between 6 and 7 weeks… Gonadal sex determination occurs around 41–42 days in human embryos, signified by the upregulation of SRY gene expression in XY embryos.”

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Women’s Healthcare in Advanced Practice Nursing keeps the sexes indistinguishable until about week 7:119

Women’s Healthcare in Advanced Practice Nursing “Up until approximately 7 weeks of embryonic development the male and female sex is indistinguishable from each other.”

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A study on Sertoli-cell hormones places Sry expression in pre-Sertoli cells at week 7:120

Lucas-Herald & Mitchell — Testicular Sertoli Cell Hormones “The Sry gene is expressed in pre-Sertoli cells at 7 weeks in the XY gonad.”

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Exploring the Dirty Side of Women’s Health again marks the end of the sixth week as the point at which the systems diverge:121

Exploring the Dirty Side of Women’s Health “At the end of the sixth week, male and female genital systems are indistinguishable in appearance… The ambisexual or indifferent phase of genital development ends at this point, and from the seventh week on the male and female systems pursue diverging pathways.”

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Effective Management of Bladder and Bowel Problems in Children describes the seventh-week embryo as still able to develop either way:122

Effective Management of Bladder and Bowel Problems in Children “The fetus at the seventh week only has genetic difference and could potentially develop either as male or female. All three components (gonads, genital ducts and external genitalia) go through a stage in which they are identical (indifferent stage) and can develop into either male or female.”

The Color Atlas of Human Fetal and Neonatal Histology dates the first step of testicular differentiation to the end of the sixth post-fertilisation week:123

Color Atlas of Human Fetal and Neonatal Histology “The first step in testicular differentiation, the transformation of the bipotential gonad into the testis, begins toward the end of the sixth postfertilization week.”

Scott Gilbert’s Developmental Biology keeps the gonad sexually indifferent until week 7:124

Scott F. Gilbert — Developmental Biology (6th ed.) “In humans, the gonadal rudiments appear in the intermediate mesoderm during week 4 and remain sexually indifferent until week 7.”

Fanaroff and Martin’s Neonatal-Perinatal Medicine places the onset of differentiation in the seventh week:125

Fanaroff & Martin’s Neonatal-Perinatal Medicine “Fetal sexual differentiation begins in the 7th week, when the bipotential gonad begins to differentiate as either a testis or an ovary.”

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Human Reproductive and Prenatal Genetics describes the gonads becoming distinguishable around the eighth gestational week:126

Qiao & Leung — Human Reproductive and Prenatal Genetics “At about 8 weeks gestation… the urogenital ridges of both sexes are no longer identical because the gonads in males will have begun to form visible testicular cords… The gonads in females form ovaries containing oocytes.”

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Here the eighth gestational week equals the sixth week from fertilisation, and the ninth equals the seventh. Diagnosis and Management of Ovarian Disorders gives the embryonic-ovary stage explicitly at day 42:127

Altchek, Deligdisch & Kase — Diagnosis and Management of Ovarian Disorders “It begins with the formation of a genital ridge at approximately 30 days post conception, followed by an indifferent gonad at 35 days, an embryonic ovary at 42 days (6 weeks), an early fetal ovary at 8 weeks.”

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Ovarian Aging makes the same contrast between fertilisation and differentiation:128

Shixuan Wang — Ovarian Aging “Although the chromosomal sex of an embryo is determined at the time of fertilization, the gonads do not begin to differentiate into testes or ovaries until weeks 6 to 7 of embryo development.”

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The Core Curriculum for Neonatal Intensive Care Nursing puts gonadal determination at week 7 and notes the fetus is undifferentiated until week 8 gestation:129

Core Curriculum for Neonatal Intensive Care Nursing “Gonadal determination with establishment of sex by 7 weeks.”

A pediatric-gynaecology text adds that the fetus carries both Wolffian and Müllerian ducts until that point:130

Good Practice in Pediatric and Adolescent Gynecology “Until 8 weeks of gestation, the human fetus is sexually undifferentiated and contains both male (Wolffian) and female (Müllerian) genital ducts.”

Campbell-Walsh-Wein Urology states that the urogenital tract is identical in both sexes before the eighth gestational week:131

Campbell-Walsh-Wein Urology “Before 8 weeks gestation, the urogenital tract is identical in the two sexes.”

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The same work describes the first six weeks as bipotential for all the relevant tissues:132

Campbell-Walsh-Wein Urology “During the first 6 weeks of embryonic development, the gonadal ridge, germ cells, internal ducts, and external genitalia are bipotential in both 46,XY and 46,XX embryos. Under the genetic influences of sex determination, the bipotential gonadal ridges differentiate into either ovaries or testes.”

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The accompanying figure illustrates this bipotential-to-differentiated transition.133

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Nine weeks counted from conception equals seven weeks of embryonic age from fertilisation. Finally, Genetic Steroid Disorders dates the initiation of ovarian and testicular differentiation to six weeks:134

Genetic Steroid Disorders “Differentiation of ovaries or testes from the bipotential gonadal ridge tissue in humans is initiated by 6 weeks.”

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A chart of disorders of sex development summarises how these pathways can go awry.

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It is thus established that both the chromosomal sex and the gonadal sex of the embryo are decided only at the end of the sixth week and the start of the seventh — exactly the moment the hadith names.

Key Genes in Sex Determination

The whole network can be summarised in a single figure of the genes that determine the embryo’s sex.135

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Many genes are necessary for determining the embryo’s sex, but all of them are ultimately governed by SRY in the male and RSPO1 in the female:136

Genetics of Disorders of Sexual Development (Monash) “Gonadal differentiation is the first step of mammalian sex determination… male sex determination is governed by SRY-dependent activation of SOX9, whereas female development involves RSPO1, an activator of the WNT/beta-catenin signaling pathway. Hormones such as androgens and anti-Müllerian hormone also affect sexual differentiation and are important for sex determination.”

Beyond the testis-promoting SRY and SOX9, other critical genes fall into two groups:137 the X-linked genes such as DAX-1, and the autosomal genes involved in gonad formation such as SF-1 and WT-1.

Genes and Mechanisms in Vertebrate Sex Determination “X-linked genes (e.g. DAX-1) and autosomal genes (e.g. SF-1 and WT1) also play critical roles in processes of sex determination and differentiation.”

A table of the most important genes affecting the embryo’s sex sets them out in full,138 as does a second table flagging specifically those whose malfunction causes sex reversal.139

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The second table marks the reversal-causing genes among them.

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The Chains of the Nuṭfa Hadith — A Ḥadīth-Methodology Study

Having established the scientific case, the author turns to the chains of transmission (ṭuruq) of the nuṭfa hadith. The aim is to demonstrate that the correct, authentic wording is the forty-two-nights formulation cited at the outset from Ṣaḥīḥ Muslim, and to clear away the confusion surrounding the various wordings — in particular the claim that the bones, flesh, hearing, sight, and sex form only on day 120. What follows is a summary map of the entire transmission.

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The Companions Who Narrated It

Three Companions narrated the hadith from the Messenger of Allah ﷺ with an authentic chain: Ibn Masʿūd, Ḥudhayfa ibn Asīd, and Abū al-Dardāʾ — in addition to Anas ibn Mālik, though his narration did not mention the time periods.

Four Companions narrated it with weak chains: Jābir ibn ʿAbdillāh, Abū Dharr, ʿAbdullāh ibn ʿAmr, and Anas ibn Mālik (in a wording other than the well-known authentic one that is free of any timing).

Only one Companion — Ibn Masʿūd — is reported as narrating it in all three of the “forties” wordings.

The “single forty” wording, by contrast, is reported from seven individuals: Ibn Masʿūd (authentic chain), Ḥudhayfa ibn Asīd (authentic chain), and Abū al-Dardāʾ (authentic chain); and Jābir ibn ʿAbdillāh, ʿAbdullāh ibn ʿAmr, Abū Dharr, and Anas ibn Mālik (all with weak chains, Anas’s being a wording other than the well-known time-free one).

The Main Wordings of the Hadith

There are four principal wordings:

The first wording — “the like of that” (mithl dhālik). The second wording — “in that, the like of that” (fī dhālik mithl dhālik). The third wording — “a single forty” (arbaʿīn wāḥida). The fourth wording — “forty days… and forty days… and forty days.”

First wording — “the like of that”Second wording — “in that, the like of that”Third wording — “a single forty”Fourth wording — “forty days ×3”
The chainsAll from al-Aʿmash, from Zayd ibn Wahb, from Ibn MasʿūdAll from al-Aʿmash, from Zayd ibn Wahb, from Ibn MasʿūdEstablished from the Companions Ḥudhayfa ibn Asīd, Abū al-Dardāʾ, and Ibn Masʿūd; and narrated through weak chains that strengthen one another from four Companions (Jābir, Abū Dharr, ʿAbdullāh ibn ʿAmr), in addition to Anas ibn Mālik (a different wording containing the timing, not the authentic time-free one)All from Zayd ibn Wahb, from Ibn Masʿūd. (Note): there are two other chains from Ibn Masʿūd — (1) Abū Wāʾil via ʿĀṣim, and (2) the chain of al-Mukhāriq ibn Sulaym from Ibn Masʿūd — neither of which is authentic; their texts differ so severely from the authentic text that they cannot be authenticated by the combined chains
The text”…then a clot the like of that, then it becomes a morsel of flesh the like of that""…then in that it becomes a clot the like of that""The nuṭfa falls into the womb for forty nights""Forty nights as a nuṭfa, forty nights as a clot, and forty nights as a morsel of flesh”

The Weak Narrators

The following narrators in the various weak chains are the points of failure.

Al-Haytham ibn Jahm — none authenticated him except Ibn Ḥibbān, who is lenient in authenticating unknown narrators, and al-Dāraquṭnī, who often authenticates and lifts the unknown status of a narrator merely on the strength of two or more reliable narrators transmitting from someone whom no one has disparaged — on condition that there is no objectionable matter (nakāra) in the text. (For al-Dāraquṭnī’s and Ibn Ḥibbān’s method in authenticating unknown narrators, see the author’s study The Method of the Ḥadīth Critics in Authenticating the Unknown Narrator, here.) This kind of authentication requires a sound text; here the text is objectionable because it mentions four forties.

Mūsā ibn Masʿūd — weak, poor in memory, not strong, “next to nothing,” prone to misreading; one who has insight into hadith does not narrate from him. Thirteen scholars of jarḥ and taʿdīl declared him weak:

The verdicts on Mūsā ibn Masʿūd Abū Aḥmad al-Ḥākim: not strong. Abū Jaʿfar al-ʿUqaylī: listed him among the weak. Abū Ḥātim al-Rāzī: truthful, but he used to misread, and there is something in some of his hadith from Sufyān. Ibn Ḥibbān: he errs. Abū ʿAbdillāh al-Ḥākim: frequently mistaken, he errs. Al-Tirmidhī: declared weak in hadith. Aḥmad ibn Ḥanbal: of the people of truthfulness, “next to nothing.” Ibn Ḥajar: truthful, poor in memory, misreads. Al-Dāraquṭnī (in al-Ḥākim’s questions): frequently mistaken, and (in al-Sulamī’s questions): he was spoken of. ʿAmr ibn ʿAlī al-Fallās: one who has insight into hadith does not narrate from him. Zakariyyā ibn Yaḥyā al-Sājī: he used to misread, and he is soft. Ibn Qāniʿ al-Baghdādī: there is weakness in him. Ibn Khuzayma: he is not used as proof. Muḥammad ibn Bashshār al-ʿAbdī: weak in hadith — I wrote much from him, then abandoned him.

ʿUbaydullāh ibn ʿAbdillāh ibn Masʿūd — did not hear from his father.

ʿAlī ibn Zayd ibn Judʿān — weak.

Aḥmad ibn ʿUbayd ibn Ismāʿīl — unknown (majhūl).

Ḥammād ibn Salama — reliable, but his memory became confused (ikhtilāṭ).

Al-Mukhāriq ibn Sulaym — his companionship is not established, and none authenticated him except Ibn Ḥibbān, who is well known for authenticating weak narrators; the authors of Taḥrīr Taqrīb al-Tahdhīb said he is “unknown — for how can his companionship be established by a hadith with a disturbed chain, over which there is so much disagreement that nothing is settled?”

ʿAbdullāh ibn al-Mukhāriq — unknown (majhūl).

ʿAbd al-Raḥmān ibn ʿAbdillāh al-Masʿūdī — reliable, but his memory became confused and changed at the end of his life; disturbed in hadith, abandoned in hadith; even Ibn Ḥibbān, who is strict in disparagement, disparaged him.

ʿĀṣim ibn ʿAlī — disputed over.

ʿĀṣim ibn Abī al-Najūd — truthful, but poor and bad in memory, disturbed in hadith, given to errors of memory, his memory became confused at the end, frequently mistaken:

The verdicts on ʿĀṣim ibn Abī al-Najūd Abū Jaʿfar al-ʿUqaylī: “there was nothing in him except poor memory.” Abū Ḥātim al-Rāzī: “righteous… his place with me is the place of truthfulness, sound in hadith, but he was not such a memoriser — it is not appropriate to say of him ‘reliable’ (thiqa).” Ismāʿīl ibn ʿUlayya: “he was poor in memory, and it is as if everyone named ʿĀṣim was poor in memory.” Ibn Ḥajar (in al-Taqrīb): “truthful, with errors of memory, a proof in recitation, and his hadith in the two Ṣaḥīḥs is paired (with another).” Al-Dāraquṭnī: “there is something in his memory.” Ḥammād ibn Salama: “ʿĀṣim’s memory became confused at the end of his life.” Muḥammad ibn Saʿd: “reliable, except that he was frequently mistaken in his hadith.” Yaḥyā ibn Saʿīd al-Qaṭṭān: “I never found a man named ʿĀṣim except that I found him poor in memory.” Yaʿqūb ibn Sufyān al-Fasawī: “in his hadith there is disturbance, though he is reliable.”

The Tābiʿūn Who Narrated the Three-Forties Wording from Ibn Masʿūd

Three followers narrated this wording from Ibn Masʿūd: Zayd ibn Wahb (authentic chain), al-Mukhāriq ibn Sulaym (not authentic), and Abū Wāʾil Shaqīq ibn Salama (not authentic).

The two chains that are not authentic from Ibn Masʿūd are as follows.

The first, from al-Sunna by al-Khallāl, runs through al-Haytham ibn Jahm and Mūsā ibn Masʿūd:

Al-Sunna by al-Khallāl 892 — Ibn Masʿūd (weak) “أَخْبَرَنَا الْحَسَنُ بْنُ عَرَفَةَ، قَالَ: حَدَّثَنِي أَبُو حُذَيْفَةَ النَّهْدِيُّ مُوسَى بْنُ مَسْعُودٍ قَالَ: ثَنَا الْهَيْثَمُ بْنُ جَهْم، عَنْ عَاصِمِ ابْنِ بَهْدَلَةَ، عَنْ أَبِي وَائِلٍ، عَنِ ابْنِ مَسْعُودٍ، أَنَّ النَّبِيَّ ﷺ قَالَ: “إِنَّ النُّطْفَةَ إِذَا اسْتَقَرَّتْ فِي الرَّحِمِ نَالَتْ كُلَّ شَعْرٍ وَبِشَرٍ، ثُمَّ تَكُونُ نُطْفَةً أَرْبَعِينَ لَيْلَةً، ثُمَّ تَكُونُ عَلَقَةً أَرْبَعِينَ لَيْلَةً، ثُمَّ تَكُونُ مُضْغَةً أَرْبَعِينَ لَيْلَةً، ثُمَّ تَكُونُ عِظَامًا أَرْبَعِينَ لَيْلَةً، ثُمَّ يَكْسُو اللَّهُ الْعَظْمَ لَحْمًا…""

“When the nuṭfa settles in the womb it reaches every hair and skin; then it is a nuṭfa for forty nights, then a clot for forty nights, then a morsel of flesh for forty nights, then bones for forty nights, then Allah clothes the bone with flesh…”

Grade: Weak — its chain turns on al-Haytham ibn Jahm and Mūsā ibn Masʿūd, and the text is objectionable because it mentions four forties.140

This chain also suffers from singularity (tafarrud). Al-Ṭabarānī notes that only al-Haytham ibn Jahm narrated it from ʿĀṣim, only Abū Ḥudhayfa narrated it from him, and al-Ḥasan ibn ʿArafa was alone in narrating it from Abū Ḥudhayfa.141 The editor of al-Khallāl’s al-Sunna, Dr. ʿAṭiyya al-Zahrānī, likewise declared it weak.142

The second, from al-Ṭabarānī’s al-Muʿjam al-Kabīr, runs through al-Masʿūdī, ʿAbdullāh ibn al-Mukhāriq, and his father al-Mukhāriq ibn Sulaym:

Al-Muʿjam al-Kabīr (al-Ṭabarānī) 9146 — Ibn Masʿūd (weak) “حَدَّثَنَا عُمَرُ بْنُ حَفْصٍ السَّدُوسِيُّ، ثنا عَاصِمُ بْنُ عَلِيٍّ، ثنا الْمَسْعُودِيُّ، عَنْ عَبْدِ اللهِ بْنِ الْمُخَارِقِ، عَنْ أَبِيهِ، قَالَ: قَالَ عَبْدُ اللهِ بْنُ مَسْعُودٍ: ”…إِنَّ النُّطْفَةَ تَكُونُ فِي الرَّحِمِ أَرْبَعِينَ، ثُمَّ تَكُونُ عَلَقَةً أَرْبَعِينَ، ثُمَّ تَكُونُ مُضْغَةً أَرْبَعِينَ، فَإِذَا أَرَادَ اللهُ أَنْ يَخْلُقَ الْخَلْقَ نَزَلَ مَلَكٌ…""

”…The nuṭfa is in the womb for forty, then a clot for forty, then a morsel of flesh for forty; and when Allah wishes to create the creation an angel descends…” Then ʿAbdullāh recited: {Indeed We created man from a sperm-drop mixture, that We may try him} [al-Insān 76:2], and said: “the ‘mixture’ (amshāj) is the veins.”

Grade: Weak — turning on al-Mukhāriq ibn Sulaym (companionship unestablished, unknown), ʿAbdullāh ibn al-Mukhāriq (unknown), ʿAbd al-Raḥmān al-Masʿūdī (confused memory), and ʿĀṣim ibn ʿAlī (disputed).143

Neither of these two can strengthen the other, because they share the same point of origin (ittiḥād al-makhraj) — both come from Ibn Masʿūd, yet with severe divergence in the text.

The Three-Forties Narrations: Enumeration of the Chains

The “three forties” material is now traced wording by wording. In what follows, every chain (silsila) shares the same upper stem — Ibn Masʿūd → Zayd ibn Wahb al-Juhanī → al-Aʿmash — unless otherwise noted; the tables list the transmitters below that stem and the collection in which each route is recorded.

Wording 1: “the like of that” (without the Word nuṭfa)

All twenty routes run from al-Aʿmash, from Zayd ibn Wahb, from Ibn Masʿūd:

#Below al-Aʿmash → … → Source
1Mashyakha ibn Ṭahmān → Musnad Aḥmad
2Jarīr ibn Abī Ḥāzim → al-Qadar by Ibn Wahb
3Zuhayr ibn Muʿāwiya → Ibn Nufayl → Mustakhraj Abī ʿAwāna
4Shuʿba → several narrators → Ṣaḥīḥ al-Bukhārī and others
5Muḥāḍir ibn al-Muwarriʿ → al-ʿAbbās al-Dūrī → Mustakhraj Abī ʿAwāna
6ʿAbd al-Razzāq → Jāmiʿ Maʿmar
7Yaḥyā ibn Zakariyyā ibn Abī Zāʾida → ʿAlī ibn al-Madīnī → ʿAbd al-Raḥmān ibn Muḥammad al-Qurashī → al-Qadar by Ibn Wahb
8Sufyān al-Thawrī → Muṣannaf ʿAbd al-Razzāq
9Muḥammad ibn ʿUbayd al-Ṭanāfusī → Musnad al-Ḥumaydī
10Abū Muʿāwiya al-Ḍarīr → Musnad Aḥmad
11(via Salama ibn Kuhayl, not al-Aʿmash) Salama ibn Kuhayl → Fiṭr ibn Khalīfa → Ḥusayn ibn Muḥammad → Musnad Aḥmad
12Yaḥyā ibn Yaʿmar → Musnad Aḥmad
13Wakīʿ ibn al-Jarrāḥ → Musnad Aḥmad
14Abū al-Aḥwaṣ → al-Ḥasan ibn al-Rabīʿ → Ṣaḥīḥ al-Bukhārī
15Ḥafṣ ibn Ghiyāth → ʿUmar ibn Ḥafṣ ibn Ghiyāth → Ṣaḥīḥ al-Bukhārī
16Muḥammad ibn ʿAbdillāh ibn Numayr → Abū Bakr ibn Abī Shayba → Ṣaḥīḥ Muslim
17Muḥammad ibn ʿUbayd al-Ṭanāfusī → Abū Muʿāwiya al-Ḍarīr → ʿAlī ibn Maymūn al-Raqqī → Sunan Ibn Mājah
18Yaḥyā ibn Saʿīd al-Qaṭṭān → Muḥammad ibn al-Muthannā → al-Sunna by Ibn Abī ʿĀṣim
19Sharīk al-Nakhaʿī → Sunan al-Nasāʾī
20al-Ḥimmānī → al-ʿAbbās al-Dūrī → al-Musnad by al-Shāshī

A large company narrated this hadith from al-Aʿmash; Ibn Wahb names many of them:144

al-Qadar by Ibn Wahb — those who narrated it from al-Aʿmash “A group narrated this hadith from al-Aʿmash, among them: Shuʿba, al-Thawrī, al-Masʿūdī, Zuhayr ibn Muʿāwiya, Khālid al-Ḥadhdhāʾ, Abū Shihāb al-Ḥannāṭ, Yaḥyā ibn Zakariyyā ibn Abī Zāʾida, Abū Muʿāwiya al-Ḍarīr, Jarīr ibn ʿAbd al-Ḥamīd, Mūsā ibn Aʿyan, ʿĪsā ibn Yūnus, Sufyān ibn ʿUyayna, ʿAmmār ibn Ruzayq, ʿAmr ibn Abī Qays, Wakīʿ ibn al-Jarrāḥ, ʿAbdullāh ibn Dāwūd, ʿAbd al-Wāḥid ibn Ziyād, Muḥammad ibn Jābir al-Suḥaymī, Saʿd ibn al-Ṣalt, and others among the shaykhs.”

Ibn Ḥajar adds that he himself collected nearly forty routes from al-Aʿmash in a dedicated treatise.145

Wording 2: “the like of that” (with the Word nuṭfa)

A single route: Ibn Masʿūd → Zayd ibn Wahb al-Juhanī → al-Aʿmash → from the hadith of Sufyān.

Wording 3: “forty Nights, and Forty Nights, and Forty nights”

This wording comes through seven routes, several of which carry the weak narrators identified earlier:

#The chain
1Ibn Masʿūd → Abū Wāʾil → ʿĀṣim ibn Bahdala → al-Haytham ibn Jahm → Mūsā ibn Masʿūd → al-Ḥasan ibn ʿArafa → al-Sunna by al-Khallāl
2Zayd ibn Wahb → al-Aʿmash → Jarīr ibn Ḥāzim → Ibn Wahb → Yūnus → Sharḥ Mushkil al-Āthār
3Zayd ibn Wahb → Salama ibn Kuhayl → Fiṭr ibn Khalīfa → Yaḥyā ibn Khallād → Bishr ibn Mūsā → Juzʾ min ḥadīth Abī ʿAlī
4al-Mukhāriq ibn Sulaym → ʿAbdullāh ibn al-Mukhāriq → ʿAbd al-Raḥmān al-Masʿūdī → ʿĀṣim ibn ʿAlī → ʿUmar ibn Ḥafṣ al-Sadūsī → al-Muʿjam al-Kabīr / Muʿjam Shuyūkh Ibn al-Aʿrābī
5Zayd ibn Wahb → al-Aʿmash → Khālid al-Ḥadhdhāʾ → Ḥammād ibn Salama → Muʾammal ibn Ismāʿīl → Aḥmad ibn ʿUbayd ibn Ismāʿīl
6Zayd ibn Wahb → Salama ibn Kuhayl → Fiṭr ibn Khalīfa → ʿUbaydullāh ibn Mūsā → Isḥāq ibn Yasār → al-Qadar by al-Firyābī
7Zayd ibn Wahb → al-Aʿmash → Warqāʾ ibn ʿUmar → Sallām ibn Sulaymān → Muḥammad ibn ʿĪsā → Khaythama ibn Sulaymān / Aḥādīth Abī al-Ḥusayn al-Kilābī

The narrators that render these routes weak are as identified above: al-Mukhāriq ibn Sulaym (companionship unestablished, unknown), ʿAbdullāh ibn al-Mukhāriq (unknown), ʿAbd al-Raḥmān al-Masʿūdī (reliable but confused in memory, disturbed, abandoned in hadith — disparaged even by Ibn Ḥibbān), ʿĀṣim ibn ʿAlī (disputed over), and Mūsā ibn Masʿūd (weak, poor memory, “next to nothing,” declared weak by thirteen critics, as detailed in the weak-narrators section above).

Wording 4: “and when the Forty pass” (fa-idhā Maḍat al-arbaʿūn)

A single route: ʿAbdullāh ibn Masʿūd → ʿUbaydullāh ibn ʿAbdillāh ibn Masʿūd → ʿAlī ibn Zayd ibn Judʿān → Hushaym ibn Bashīr → Musnad Aḥmad. This route is weak: ʿUbaydullāh did not hear from his father, and ʿAlī ibn Zayd ibn Judʿān is weak.

Wording 5: “then in that it becomes… the like of that” (thumma Yakūnu Fī dhālik… Mithl dhālik)

From Ibn Masʿūd → Zayd ibn Wahb → al-Aʿmash, branching to four narrators — Wakīʿ, Abū Muʿāwiya, Muḥammad ibn ʿAbdillāh ibn Numayr, and ʿAbdullāh ibn Numayr — all recorded in Ṣaḥīḥ Muslim.

The Three-Forties Narrations: Index of Chains

Before the full narration texts, the author lays out an index of every chain (ṭarīq) for each wording, to show how the transmission is distributed. Unless otherwise noted, every chain shares the common stem: Ibn Masʿūd ← Zayd ibn Wahb al-Juhanī ← al-Aʿmash.

Wording “the like of that” (without the word nuṭfa) — twenty chains

#From al-Aʿmash (or from Zayd ibn Wahb) onwardSource
1Mishyakha ibn Ṭahmān
2Jarīr ibn Abī Ḥāzim → Ibn Wahbal-Qadar by Ibn Wahb
3Zuhayr ibn Muʿāwiya → Ibn NufaylMustakhraj Abī ʿAwāna
4Shuʿba → several narratorsṢaḥīḥ al-Bukhārī and others
5Muḥāḍir ibn al-Muwarriʿ → ʿAbbās al-DūrīMustakhraj Abī ʿAwāna
6ʿAbd al-Razzāq (← Maʿmar)al-Jāmiʿ of Maʿmar
7Yaḥyā ibn Zakariyyā ibn Abī Zāʾida → ʿAlī ibn al-Madīnī → ʿAbd al-Raḥmān ibn Muḥammad al-Qurashīal-Qadar by Ibn Wahb
8Sufyān al-ThawrīMuṣannaf ʿAbd al-Razzāq
9Muḥammad ibn ʿUbayd al-ṬanāfisīMusnad al-Ḥumaydī
10Abū Muʿāwiya al-ḌarīrMusnad Aḥmad
11(via Zayd ibn Wahb ←) Salama ibn Kuhayl → Fiṭr ibn Khalīfa → Ḥusayn ibn MuḥammadMusnad Aḥmad
12Yaḥyā ibn YaʿmarMusnad Aḥmad
13Wakīʿ ibn al-JarrāḥMusnad Aḥmad
14Abū al-Aḥwaṣ → al-Ḥasan ibn al-RabīʿṢaḥīḥ al-Bukhārī
15Ḥafṣ ibn Ghiyāth → ʿUmar ibn Ḥafṣ ibn GhiyāthṢaḥīḥ al-Bukhārī
16Muḥammad ibn ʿAbdillāh ibn Numayr → Abū Bakr ibn Abī ShaybaṢaḥīḥ Muslim
17Muḥammad ibn ʿUbayd al-Ṭanāfisī / Abū Muʿāwiya al-Ḍarīr → ʿAlī ibn Maymūn al-RaqqīSunan Ibn Mājah
18Yaḥyā ibn Saʿīd al-Qaṭṭān → Muḥammad ibn al-Muthannāal-Sunna by Ibn Abī ʿĀṣim
19Sharīk al-NakhaʿīSunan al-Nasāʾī
20al-Ḥimmānī → ʿAbbās al-Dūrīal-Musnad by al-Shāshī

A great many narrators transmitted this hadith from al-Aʿmash. Ibn Wahb records:144

Ibn Wahb — al-Qadar “This hadith was narrated from al-Aʿmash by a group, among them: Shuʿba, al-Thawrī, al-Masʿūdī, Zuhayr ibn Muʿāwiya, Khālid al-Ḥadhdhāʾ, Abū Shihāb al-Ḥannāṭ, Yaḥyā ibn Zakariyyā ibn Abī Zāʾida, Abū Muʿāwiya al-Ḍarīr, Jarīr ibn ʿAbd al-Ḥamīd, Mūsā ibn Aʿyan, ʿĪsā ibn Yūnus, Sufyān ibn ʿUyayna, ʿAmmār ibn Ruzayq, ʿAmr ibn Abī Qays, Wakīʿ ibn al-Jarrāḥ, ʿAbdullāh ibn Dāwūd, ʿAbd al-Wāḥid ibn Ziyād, Muḥammad ibn Jābir al-Suḥaymī, Saʿd ibn al-Ṣalt, and others among the shaykhs.”

Ibn Ḥajar adds that he himself compiled the chains in a separate booklet: “I had documented it in a juzʾ comprising about forty separate chains from al-Aʿmash.”145

Wording “the like of that” (with the word nuṭfa)

This comes through the single line: Ibn Masʿūd ← Zayd ibn Wahb al-Juhanī ← al-Aʿmash, from the hadith of Sufyān.

Wording “forty nights… and forty nights… and forty nights” — seven chains

#ChainSource
1Abū Wāʾil ← ʿĀṣim ibn Bahdala ← al-Haytham ibn Jahm ← Mūsā ibn Masʿūd ← al-Ḥasan ibn ʿArafaal-Sunna by al-Khallāl
2Zayd ibn Wahb ← al-Aʿmash ← Jarīr ibn Ḥāzim ← Ibn Wahb ← YūnusSharḥ Mushkil al-Āthār
3Zayd ibn Wahb ← Salama ibn Kuhayl ← Fiṭr ibn Khalīfa ← Yaḥyā ibn Khallād ← Bishr ibn MūsāJuzʾ min ḥadīth Abī ʿAlī
4al-Mukhāriq ibn Sulaym ← ʿAbdullāh ibn al-Mukhāriq ← ʿAbd al-Raḥmān al-Masʿūdī ← ʿĀṣim ibn ʿAlī ← ʿUmar ibn Ḥafṣ al-Sadūsīal-Muʿjam al-Kabīr (al-Ṭabarānī); Muʿjam Shuyūkh Ibn al-Aʿrābī
5Zayd ibn Wahb ← al-Aʿmash ← Khālid al-Ḥadhdhāʾ ← Ḥammād ibn Salama ← Muʾammal ibn Ismāʿīl(collection of) Aḥmad ibn ʿUbayd ibn Ismāʿīl
6Zayd ibn Wahb ← Salama ibn Kuhayl ← Fiṭr ibn Khalīfa ← ʿUbaydullāh ibn Mūsā ← Isḥāq ibn Yasāral-Qadar by al-Firyābī
7Zayd ibn Wahb ← al-Aʿmash ← Waraqāʾ ibn ʿUmar ← Sallām ibn Sulaymān ← Muḥammad ibn ʿĪsāKhaythama ibn Sulaymān; Aḥādīth Abī al-Ḥusayn al-Kilābī

These three-forties chains fail on the weak narrators detailed earlier: al-Mukhāriq ibn Sulaym (companionship unestablished, unknown), ʿAbdullāh ibn al-Mukhāriq (unknown), ʿAbd al-Raḥmān al-Masʿūdī (confused memory, disturbed and abandoned in hadith — disparaged even by the strict Ibn Ḥibbān), ʿĀṣim ibn ʿAlī (disputed over), and Mūsā ibn Masʿūd (weak, poor in memory — declared weak by thirteen critics, as set out above).

Wording “when the forty has passed”

A single chain: ʿAbdullāh ibn Masʿūd ← ʿUbaydullāh ibn ʿAbdillāh ibn Masʿūd ← ʿAlī ibn Zayd ibn Judʿān ← Hushaym ibn Bashīr, recorded in Musnad Aḥmad — and it fails on the weak ʿAlī ibn Zayd ibn Judʿān and the broken link of ʿUbaydullāh, who did not hear from his father.

Wording “then in that it becomes a clot the like of that”

Through the common stem Ibn Masʿūd ← Zayd ibn Wahb ← al-Aʿmash, narrated onward by Wakīʿ, Abū Muʿāwiya, Muḥammad ibn ʿAbdillāh ibn Numayr, and ʿAbdullāh ibn Numayr — recorded in Ṣaḥīḥ Muslim.

The Narrations

What follows are the narration texts themselves. The sheer number of chains — all converging on al-Aʿmash, from Zayd ibn Wahb, from Ibn Masʿūd, and spread across Bukhārī, Muslim, Aḥmad, al-Ḥumaydī, al-Ṭayālisī, ʿAbd al-Razzāq, Abū ʿAwāna, Ibn Abī ʿĀṣim, al-Nasāʾī, al-Shāshī, and more — establishes the wording beyond doubt.

The Ṣaḥīḥayn Wordings

The foundation is the version in Ṣaḥīḥ al-Bukhārī, through Shuʿba, from al-Aʿmash:

Ṣaḥīḥ al-Bukhārī 6594 — Ibn Masʿūd (RA) «إِنَّ أَحَدَكُمْ يُجْمَعُ فِي بَطْنِ أُمِّهِ أَرْبَعِينَ يَوْمًا، ثُمَّ عَلَقَةً مِثْلَ ذَلِكَ، ثُمَّ يَكُونُ مُضْغَةً مِثْلَ ذَلِكَ، ثُمَّ يَبْعَثُ اللهُ مَلَكًا فَيُؤْمَرُ بِأَرْبَعٍ: بِرِزْقِهِ وَأَجَلِهِ، وَشَقِيٌّ أَوْ سَعِيدٌ…»

“Each of you is gathered in his mother’s womb for forty days, then a clot for the like of that, then he becomes a morsel of flesh for the like of that; then Allah sends an angel who is commanded with four matters: his provision, his lifespan, and whether wretched or blessed…”

Grade: Ṣaḥīḥ · al-Bukhārī (Shuʿba ← al-Aʿmash)146

Muslim records it through Abū Muʿāwiya, Wakīʿ, and Ibn Numayr — with the distinctive phrasing “in that” (fī dhālik):

Ṣaḥīḥ Muslim 2643 — Ibn Masʿūd (RA) «إِنَّ أَحَدَكُمْ يُجْمَعُ خَلْقُهُ فِي بَطْنِ أُمِّهِ أَرْبَعِينَ يَوْمًا، ثُمَّ يَكُونُ فِي ذَلِكَ عَلَقَةً مِثْلَ ذَلِكَ، ثُمَّ يَكُونُ فِي ذَلِكَ مُضْغَةً مِثْلَ ذَلِكَ، ثُمَّ يُرْسَلُ الْمَلَكُ فَيَنْفُخُ فِيهِ الرُّوحَ…»

“The creation of each of you is gathered in his mother’s womb for forty days, then in that he becomes a clot for the like of that, then in that a morsel of flesh for the like of that, then the angel is sent and breathes the spirit into him…”

Grade: Ṣaḥīḥ · Muslim147

The Explicit Three-Forties Wordings

Several authentic chains state the three forties outright — forty nights as a nuṭfa, forty as a clot, forty as a morsel of flesh. Ibn Wahb records, via Jarīr ibn Ḥāzim from al-Aʿmash:

al-Qadar by Ibn Wahb 37 — Ibn Masʿūd (RA) «تَكُونُ النُّطْفَةُ فِي الرَّحِمِ أَرْبَعِينَ لَيْلَةً نُطْفَةً، وَأَرْبَعِينَ لَيْلَةً عَلَقَةً، وَأَرْبَعِينَ لَيْلَةً مُضْغَةً، ثُمَّ يَبْعَثُ إِلَيْهَا مَلَكًا فَيُؤْمَرُ بِأَرْبَعِ كَلِمَاتٍ: بِرِزْقِهِ، وَأَجَلِهِ، وَأَثَرِهِ، وَشَقِيٍّ أَمْ سَعِيدٍ…»

“The nuṭfa is in the womb forty nights as a nuṭfa, forty nights as a clot, and forty nights as a morsel of flesh; then He sends to it an angel who is commanded with four matters: its provision, its lifespan, its deeds, and whether wretched or blessed…”

Grade: Ṣaḥīḥ chain (Jarīr ibn Ḥāzim ← al-Aʿmash ← Zayd ibn Wahb)148

Al-Ṭaḥāwī gives the same in Sharḥ Mushkil al-Āthār, through Ibn Wahb from Jarīr:

Sharḥ Mushkil al-Āthār 3870 — Ibn Masʿūd (RA) «تَكُونُ النُّطْفَةُ فِي الرَّحِمِ أَرْبَعِينَ لَيْلَةً نُطْفَةً، وَأَرْبَعِينَ لَيْلَةً عَلَقَةً، وَأَرْبَعِينَ لَيْلَةً مُضْغَةً، ثُمَّ يُبْعَثُ إِلَيْهِ مَلَكٌ، فَيُؤْمَرُ بِأَرْبَعِ كَلِمَاتٍ…»

Grade: Ṣaḥīḥ chain149

Abū ʿAlī al-Ṣawwāf records it via Salama ibn Kuhayl, from Fiṭr:

Juzʾ Abī ʿAlī al-Ṣawwāf 46 — Ibn Masʿūd (RA) «يُجْمَعُ خَلْقُ أَحَدِكُمْ فِي بَطْنِ أُمِّهِ أَرْبَعِينَ لَيْلَةً، ثُمَّ يَكُونُ نُطْفَةً أَرْبَعِينَ، ثُمَّ يَكُونُ عَلَقَةً أَرْبَعِينَ، ثُمَّ يَكُونُ مُضْغَةً أَرْبَعِينَ، ثُمَّ يُبْعَثُ إِلَيْهِ مَلَكٌ…»

Grade: Ṣaḥīḥ chain (Salama ibn Kuhayl ← Fiṭr ← Zayd ibn Wahb)150

Al-Firyābī’s al-Qadar, through ʿUbaydullāh ibn Mūsā from Fiṭr, has the three forties as days:

al-Qadar by al-Firyābī 127 — Ibn Masʿūd (RA) «يُجْمَعُ خَلْقُ أَحَدِكُمْ فِي بَطْنِ أُمِّهِ أَرْبَعِينَ يَوْمًا، ثُمَّ يَكُونُ عَلَقَةً أَرْبَعِينَ يَوْمًا، ثُمَّ يَكُونُ مُضْغَةً أَرْبَعِينَ يَوْمًا، ثُمَّ يَبْعَثُ اللهُ لَهُ مَلَكًا…»

Grade: Ṣaḥīḥ chain151

Ibn al-Aʿrābī records it through Ḥammād ibn Salama, from Khālid al-Ḥadhdhāʾ, from al-Aʿmash:

Muʿjam Shuyūkh Ibn al-Aʿrābī 976 — Ibn Masʿūd (RA) «إِنَّ خَلْقَ أَحَدِكُمْ يُجْمَعُ فِي بَطْنِ أُمِّهِ، فَيَكُونُ نُطْفَةً أَرْبَعِينَ يَوْمًا، ثُمَّ يَكُونُ عَلَقَةً أَرْبَعِينَ يَوْمًا، ثُمَّ يَكُونُ مُضْغَةً أَرْبَعِينَ يَوْمًا، ثُمَّ يَبْعَثُ اللهُ إِلَيْهِ الْمَلَكَ…»

Grade: Ḥasan chain152

And al-Kilābī (recorded by Khaythama), via Waraqāʾ from al-Aʿmash, adds the closing exhortation to act:

Aḥādīth Abī al-Ḥusayn al-Kilābī 61 — Ibn Masʿūd (RA) «أَنَّ خَلْقَ أَحَدِكُمْ يُجْمَعُ فِي بَطْنِ أُمِّهِ فَيَكُونُ نُطْفَةً أَرْبَعِينَ يَوْمًا، ثُمَّ يَكُونُ عَلَقَةً أَرْبَعِينَ، ثُمَّ يَكُونُ مُضْغَةً أَرْبَعِينَ… قَالَ: بَلِ اعْمَلُوا فَكُلٌّ مُيَسَّرٌ لِمَا خُلِقَ لَهُ»

“…they said: ‘O Messenger of Allah, shall we not then rely on it and not act?’ He said: ‘Rather, act, for everyone is made easy for that which he was created.’”

Grade: Ṣaḥīḥ chain153

Further Corroborating Chains (Standard Wording)

The remaining chains carry the well-known “the like of that” wording and reinforce the same transmission. From the hadith of Sufyān, the nuṭfa is given as forty nights:154

From the hadith of Sufyān 292 — Ibn Masʿūd (RA) «إِنَّ خَلْقَ أَحَدِكُمْ يُجْمَعُ فِي بَطْنِ أُمِّهِ أَرْبَعِينَ لَيْلَةً نُطْفَةً، ثُمَّ يَكُونُ عَلَقَةً مِثْلَ ذَلِكَ، ثُمَّ يَكُونُ مُضْغَةً مِثْلَ ذَلِكَ…»

Ibn al-Jaʿd, through Zuhayr from al-Aʿmash:155

Musnad Ibn al-Jaʿd 2594 — Ibn Masʿūd (RA) «إِنَّ خَلْقَ أَحَدِكُمْ يُجْمَعُ فِي بَطْنِ أُمِّهِ أَرْبَعِينَ يَوْمًا، ثُمَّ يَكُونُ نُطْفَةً مِثْلَ ذَلِكَ، ثُمَّ يَكُونُ عَلَقَةً مِثْلَ ذَلِكَ، ثُمَّ يَكُونُ مُضْغَةً مِثْلَ ذَلِكَ…»

The same wording recurs across the collections, each through al-Aʿmash from Zayd ibn Wahb (unless noted). Abū ʿAwāna records two routes (Wahb ibn Jarīr ← Shuʿba; and Muḥāḍir, giving “forty nights”);156157 Mishyakha Ibn Ṭahmān (no. 82) abridges it;158 Ibn Wahb’s al-Qadar (no. 39) gives ʿAlī ibn al-Madīnī’s wording via Yaḥyā ibn Zakariyyā;159 al-Ṭayālisī (no. 296) through Shuʿba;160 ʿAbd al-Razzāq (no. 21160, and again 20093) through al-Thawrī;161162 al-Ḥumaydī (no. 126) through Muḥammad ibn ʿUbayd al-Ṭanāfisī;163 and Aḥmad records it repeatedly — through Abū Muʿāwiya (3624), through Ḥusayn ibn Muḥammad from Fiṭr (3934), and through Yaḥyā and Wakīʿ (4091).164165166

Musnad Aḥmad 3624 — Ibn Masʿūd (RA) «إِنَّ أَحَدَكُمْ يُجْمَعُ خَلْقُهُ فِي بَطْنِ أُمِّهِ فِي أَرْبَعِينَ يَوْمًا، ثُمَّ يَكُونُ عَلَقَةً مِثْلَ ذَلِكَ، ثُمَّ يَكُونُ مُضْغَةً مِثْلَ ذَلِكَ، ثُمَّ يُرْسَلُ إِلَيْهِ الْمَلَكُ، فَيَنْفُخُ فِيهِ الرُّوحَ، وَيُؤْمَرُ بِأَرْبَعِ كَلِمَاتٍ…»

Grade: Ṣaḥīḥ164

Al-Bukhārī himself records it again twice more — through Abū al-Aḥwaṣ (3208) and through ʿUmar ibn Ḥafṣ from his father (3332);167168 Ibn Abī ʿĀṣim in al-Sunna (no. 175) through Yaḥyā al-Qaṭṭān;169 al-Nasāʾī in al-Sunan al-Kubrā (11182) through Fiṭr and through Sharīk;170 al-Khallāl (no. 890) through Muḥammad ibn ʿUbayd al-Ṭanāfisī;171 al-Shāshī (no. 680) through al-Ḥimmānī;172 Maʿmar’s al-Jāmiʿ (20093);162 and Ibn Mājah (no. 76) through Wakīʿ, Ibn Fuḍayl, and Abū Muʿāwiya.173

Ṣaḥīḥ al-Bukhārī 3332 — Ibn Masʿūd (RA) «إِنَّ أَحَدَكُمْ يُجْمَعُ فِي بَطْنِ أُمِّهِ أَرْبَعِينَ يَوْمًا ثُمَّ يَكُونُ عَلَقَةً مِثْلَ ذَلِكَ ثُمَّ يَكُونُ مُضْغَةً مِثْلَ ذَلِكَ ثُمَّ يَبْعَثُ اللهُ إِلَيْهِ مَلَكًا بِأَرْبَعِ كَلِمَاتٍ…»

Grade: Ṣaḥīḥ168

The “When the Forty Has Passed” Variant

One narration in Musnad Aḥmad, through the weak ʿAlī ibn Zayd from Abū ʿUbayda, carries a distinctive wording that explicitly lists the sex of the embryo among what the angel records:

Musnad Aḥmad 3553 — Ibn Masʿūd (RA) (weak) «إِنَّ النُّطْفَةَ تَكُونُ فِي الرَّحِمِ أَرْبَعِينَ يَوْمًا عَلَى حَالِهَا لَا تَغَيَّرُ، فَإِذَا مَضَتِ الْأَرْبَعُونَ صَارَتْ عَلَقَةً، ثُمَّ مُضْغَةً كَذَلِكَ، ثُمَّ عِظَامًا كَذَلِكَ، فَإِذَا أَرَادَ اللهُ أَنْ يُسَوِّيَ خَلْقَهُ، بَعَثَ إِلَيْهَا مَلَكًا، فَيَقُولُ: أَيْ رَبِّ، أَذَكَرٌ أَمْ أُنْثَى؟ أَشَقِيٌّ أَمْ سَعِيدٌ؟…»

“The nuṭfa remains in the womb forty days unchanged; when the forty has passed it becomes a clot, then a morsel of flesh likewise, then bones likewise; and when Allah wishes to fashion its form, He sends to it an angel who says: ‘O Lord, male or female? wretched or blessed?…’”

Grade: Weak (ʿAlī ibn Zayd ibn Judʿān; ʿUbaydullāh did not hear from his father)174

The Weak Chains

For completeness, two weak chains carry the four-forties wording with the explicit mention of bones. The first is the al-Khallāl narration through al-Haytham ibn Jahm and Mūsā ibn Masʿūd (no. 892), and the second is al-Ṭabarānī’s through al-Mukhāriq (no. 9146) — both detailed earlier among the weak narrators.175176 A further isolated weak chain runs through Sallām al-Ṭawīl, from Zayd al-ʿAmmī, from Ḥammād, from Abū Wāʾil:

Via Sallām al-Ṭawīl — Ibn Masʿūd (RA) (weak) «إِنَّ النُّطْفَةَ لَتَكُونُ فِي الرَّحِمِ أَرْبَعِينَ يَوْمًا، ثُمَّ تَكُونُ مِثْلَ ذَلِكَ عَلَقَةً، ثُمَّ تَكُونُ مِثْلَ ذَلِكَ مُضْغَةً…»

Grade: Weak (Sallām al-Ṭawīl)

The Forty-Two-Night Narrations

These are the narrations carrying the single-forty wording — the forty-two-night formulation that is the basis of this entire study. It is reported from six Companions: Ḥudhayfa ibn Asīd, Ibn Masʿūd, Abū al-Dardāʾ, Jābir, Abū Dharr, and Anas.

Index of Chains

The narrations of Ḥudhayfa ibn Asīd al-Ghifārī all pass through Abū al-Ṭufayl (ʿĀmir ibn Wāthila):

Chain (← Abū al-Ṭufayl ← Ḥudhayfa)Source
ʿIkrima ibn Khālid ← ʿAbdullāh ibn ʿAṭāʾ ← Abū Khaythama Zuhayr ← Yaḥyā ibn Abī BukayrṢaḥīḥ Muslim
Abū Kulthūm ibn Jabr ← Rabīʿa ibn Kulthūm ← ʿAbd al-Ṣamad ibn ʿAbd al-WārithṢaḥīḥ Muslim
Yūsuf al-Makkī ← ʿAzra ibn Thābit al-Anṣārī ← Abū ʿAwāna ← Muʿtamir ibn Sulaymānal-Sunna by Ibn Abī ʿĀṣim
ʿAmr ibn Dīnār ← Sufyān ibn ʿUyayna ← Ibn Kāsibal-Sunna by Ibn Abī ʿĀṣim
(Abū al-Zubayr ← Ibn Jurayj ← Rawḥ ibn ʿUbāda)al-Qadar by Ibn Wahb
ʿUbaydullāh ibn Abī Ṭalḥa al-Makkī ← Yazīd ibn Abī Ḥabīb ← Ibn Lahīʿaal-Qadar by Ibn Wahb
ʿAbdullāh ibn ʿUthmān ibn Khuthaym ← Ibn ʿAyyāsh ← Marwān ibn Muḥammad al-AsadīFawāʾid Tammām

The single-forty wording from the other five Companions is distributed as follows: Ibn Masʿūd through al-Aʿmash, from Mālik ibn al-Ḥārith, recorded in al-Bukhārī’s al-Adab al-Mufrad; Jābir through Abū al-Zubayr, recorded in Ibn Baṭṭa’s al-Ibāna al-Kubrā; Anas through ʿUbaydullāh ibn Abī Bakr, recorded in al-Firyābī’s al-Qadar; Abū Dharr through Abū Tamīm al-Jayshānī and Ibn Lahīʿa, recorded in al-Qadar by Ibn Wahb; Abū al-Dardāʾ through Abū Sallām, recorded in al-Ibāna al-Kubrā; and ʿAbdullāh ibn ʿAmr through ʿĪsā ibn Hilāl and Ibn Lahīʿa, recorded in al-ʿUluww by al-Dhahabī. Two narrators in these chains should be noted: Abū al-Zubayr al-Makkī (reliable, but a concealer of chains — mudallis), and Ibn Lahīʿa (weak).

The Narrations

The anchor of the whole study is the narration of Ḥudhayfa ibn Asīd in Ṣaḥīḥ Muslim, through Abū al-Zubayr from Abū al-Ṭufayl — the explicit forty-two-night wording naming hearing, sight, skin, flesh, and bones, and the question of sex:

Ṣaḥīḥ Muslim 2645 — Ḥudhayfa ibn Asīd (RA) «إِذَا مَرَّ بِالنُّطْفَةِ ثِنْتَانِ وَأَرْبَعُونَ لَيْلَةً بَعَثَ اللهُ إِلَيْهَا مَلَكًا، فَصَوَّرَهَا، وَخَلَقَ سَمْعَهَا وَبَصَرَهَا وَجِلْدَهَا وَلَحْمَهَا وَعِظَامَهَا، ثُمَّ قَالَ: يَا رَبِّ أَذَكَرٌ أَمْ أُنْثَى؟ فَيَقْضِي رَبُّكَ مَا شَاءَ، وَيَكْتُبُ الْمَلَكُ…»

“When forty-two nights have passed over the nuṭfa, Allah sends to it an angel who forms it and creates its hearing, its sight, its skin, its flesh, and its bones. Then he says: ‘O Lord, male or female?’ and your Lord decrees what He wills and the angel records it…”

Grade: Ṣaḥīḥ · Muslim177

Muslim also records two further wordings of Ḥudhayfa’s hadith — Zuhayr’s, which adds “well-formed or not well-formed,” and Rabīʿa’s, which gives the number as “forty-odd nights” (biḍʿin wa-arbaʿīn):

Ṣaḥīḥ Muslim 2645 — Ḥudhayfa ibn Asīd (RA), other wordings «إِنَّ النُّطْفَةَ تَقَعُ فِي الرَّحِمِ أَرْبَعِينَ لَيْلَةً، ثُمَّ يَتَصَوَّرُ عَلَيْهَا الْمَلَكُ… فَيَقُولُ: يَا رَبِّ أَذَكَرٌ أَوْ أُنْثَى؟… يَا رَبِّ أَسَوِيٌّ أَوْ غَيْرُ سَوِيٍّ؟…» — وفي رواية: «أَنَّ مَلَكًا مُوَكَّلًا بِالرَّحِمِ إِذَا أَرَادَ اللهُ أَنْ يَخْلُقَ شَيْئًا بِإِذْنِ اللهِ لِبِضْعٍ وَأَرْبَعِينَ لَيْلَةً…»

Grade: Ṣaḥīḥ · Muslim178

A second important narration of Ḥudhayfa’s, in Ibn Abī ʿĀṣim’s al-Sunna, lists what the angel forms — bone, flesh, blood, hair, skin, hearing, and sight — alongside the sex, and gives the number as forty or forty-five nights:

al-Sunna by Ibn Abī ʿĀṣim 179 — Ḥudhayfa ibn Asīd (RA) «إِنَّ النُّطْفَةَ إِذَا وَقَعَتْ فِي الرَّحِمِ أَرْبَعِينَ لَيْلَةً — وَقَالَ أَصْحَابِي: خَمْسًا وَأَرْبَعِينَ لَيْلَةً — … فَيَجِيءُ مَلَكُ الرَّحِمِ فَيَدْخُلُ فَيُصَوِّرُ لَهُ عَظْمَهُ، وَلَحْمَهُ، وَدَمَهُ، وَشَعْرَهُ، وَبَشَرَهُ، وَسَمْعَهُ، وَبَصَرَهُ، ثُمَّ يَقُولُ: أَيْ رَبِّ، أَذَكَرٌ أَمْ أُنْثَى؟…»

Grade: Ṣaḥīḥ chain179

Ibn Abī ʿĀṣim records it again through ʿAmr ibn Dīnār from Abū al-Ṭufayl, with the same “forty or forty-five” figure:

al-Sunna by Ibn Abī ʿĀṣim 180 — Ḥudhayfa ibn Asīd (RA) «يَدْخُلُ مَلَكُ الْأَرْحَامِ عَلَى النُّطْفَةِ بَعْدَمَا تَسْتَقِرُّ فِي الرَّحِمِ أَرْبَعِينَ — أَوْ قَالَ: خَمْسَةً وَأَرْبَعِينَ — فَيَقُولُ: أَيْ رَبِّ، أَشَقِيٌّ أَمْ سَعِيدٌ؟… أَيْ رَبِّ، أَذَكَرٌ أَمْ أُنْثَى؟…»

Grade: Ṣaḥīḥ chain180

The wording is corroborated from Ibn Masʿūd in al-Bukhārī’s al-Adab al-Mufrad — graded by al-Albānī as a sound chain in the suspended (mawqūf) form, though the portion beginning “Indeed the nuṭfa…” takes the ruling of the elevated (marfūʿ) and is authentically established as such:181

al-Adab al-Mufrad 283 — Ibn Masʿūd (RA) «إِنَّ النُّطْفَةَ لَتَسْتَقِرُّ فِي الرَّحِمِ أَرْبَعِينَ لَيْلَةً، ثُمَّ تَنْحَدِرُ دَمًا، ثُمَّ تَكُونُ عَلَقَةً، ثُمَّ تَكُونُ مُضْغَةً، ثُمَّ يَبْعَثُ اللَّهُ مَلَكًا فَيُكْتَبُ رِزْقُهُ وَخُلُقُهُ، وَشَقِيًّا أَوْ سَعِيدًا»

Grade: Sound chain (mawqūf, with marfūʿ ruling)181

From Abū al-Dardāʾ, in Ibn Baṭṭa’s al-Ibāna al-Kubrā, in the report of Kaʿb to Muʿāwiya:182

al-Ibāna al-Kubrā 1817 — Abū al-Dardāʾ (RA) «إِنَّ اللَّهَ لَمْ يَدَعْ نَفْسًا حِينَ تَسْتَقِرُّ نُطْفَتُهَا فِي الرَّحِمِ أَرْبَعِينَ لَيْلَةً إِلَّا كَتَبَ خَلْقَهَا وَخُلُقَهَا وَأَجَلَهَا وَرِزْقَهَا…»

Grade: Ḥasan182

From Jābir ibn ʿAbdillāh, also in al-Ibāna al-Kubrā, giving “forty days or forty nights”:183

al-Ibāna al-Kubrā 1405 — Jābir ibn ʿAbdillāh (RA) «إِذَا وَقَعَتِ النُّطْفَةُ فِي الرَّحِمِ، مَكَثَتْ فِيهِ أَرْبَعِينَ يَوْمًا أَوْ أَرْبَعِينَ لَيْلَةً، فَإِذَا أَذِنَ اللَّهُ بِخَلْقِهَا، قَالَ الْمَلَكُ: رَبِّ أَجَلُهُ؟… رَبِّ شَقِيٌّ أَوْ سَعِيدٌ؟…»

Grade: Weak (Ibn Lahīʿa)183

From Abū Dharr, in al-Qadar by Ibn Wahb:184

al-Qadar by Ibn Wahb 36 — Abū Dharr (RA) «إِذَا دَخَلَتْ — يَعْنِي: النُّطْفَة — فِي الرَّحِمِ أَرْبَعِينَ لَيْلَةً؛ أَتَى مَلَكُ النَّفْسِ، فَعَرَجَ إِلَى الرَّبِّ، فَقَالَ: يَا رَبِّ، عَبْدُكَ أَذَكَرٌ أَمْ أُنْثَى؟… أَشَقِيٌّ أَمْ سَعِيدٌ؟…»

Grade: Weak (Ibn Lahīʿa)184

And from Anas, in al-Firyābī’s al-Qadar:185

al-Qadar by al-Firyābī 145 — Anas ibn Mālik (RA) «إِنَّ النُّطْفَةَ تَكُونُ فِي الرَّحِمِ أَرْبَعِينَ لَيْلَةً… فَإِذَا أَرَادَ أَنْ يَخْلُقَهَا، قَالَ الْمَلَكُ: يَا رَبِّ، أَذَكَرٌ أَمْ أُنْثَى؟ أَشَقِيٌّ أَمْ سَعِيدٌ؟ مَا أَجَلُهُ؟ مَا رِزْقُهُ؟…»

Grade: Weak185

Statements of the Scholars

There is near-consensus among the scholars that the spirit is breathed in after 120 days (three forties).186 As for the time at which hearing, sight, flesh, and bone are created and the sex is determined, the majority of the scholars hold that this occurs after the first forty and at the start of the second (forty-odd nights).187

Ibn Ḥajar shows that he was unmoved by many of the reconciliation attempts produced by earlier scholars, remarking that most of them were assertions without evidence:

Ibn Ḥajar — Fatḥ al-Bārī “Thus he said, but holding to the apparent meaning of the reports is more appropriate; most of what has been transmitted from these scholars is assertions without any indication to support them.”

The author’s survey of scholars divides into two groups: those who place the organ-creation and sex-determination in the third forty (and whose reconciliations the author critiques), and those who place it at the end of the first forty and beginning of the second (his preferred reading).

Group One: Those Who Place Organ-Creation in the Third Forty

1 — al-Qāḍī ʿIyāḍ (544 AH)

al-Qāḍī ʿIyāḍ — Ikmāl al-Muʿlim “This is the interpretation of what has come in the hadith in its various wordings. The meaning of ‘nuṭfa’ in this context is its formation, and ‘creating its hearing and sight’ means recording that and what Allah decreed from it — as proved by what follows: ‘male or female?’ and in another narration ‘well-formed or otherwise?’”

Al-Qāḍī attempted to reconcile the Ḥudhayfa and Ibn Masʿūd hadiths by holding that the creation of hearing, sight, flesh, bone, and sex determination occurs at the start of the second forty — but that this is a recording (planning), with actual formation happening only at the end of the third forty. His argument was based on medical observation of miscarried fetuses, and on the scholarly consensus that ensoulment does not occur before four months. Ibn Ḥajar, however, records an objection to his position:

Ibn Ḥajar — Fatḥ al-Bārī (rebuttal of al-Qāḍī) “His claim that formation in truth only occurs in the third forty has been challenged, since formation has been observed in many fetuses in the second forty, as has the distinction of male from female.”

The author’s additional rebuttals: (a) the Arabic word khalaqa (created/formed) does not mean kataba (recorded/wrote) — no lexical basis supports that equivalence; (b) al-Qāḍī’s medical observation is overridden by what modern embryology has now established with certainty — that bone ossification and sex determination both begin at the end of the sixth week (42 nights); (c) the consensus cited (ensoulment after 120 days) is precisely that — consensus on ensoulment, not on the creation of the organs — the two things are distinct; (d) the thumma (then) in the hadith does not require a delay of a full 80 days; it could equally mean a delay of an hour or a day.

2 — Ibn al-Ṣalāḥ (643 AH)

Ibn al-Ṣalāḥ — cited in Fatḥ al-Bārī “The way to reconcile between the two [hadiths] is to construe the sending of the angel as multiple — once at the start of the second forty, and again at the end of the third forty for the breathing of the spirit.”

He elsewhere acknowledges that some later commentators held that the creation and formation takes place in the latter part of the second forty in reality, and that there is nothing in Ibn Masʿūd’s hadith to contradict this.188

The author replies: no narration in any chain states that the angel came twice. Positing multiple descents is an assertion without evidence and does not in any case resolve the conflict — for if the first angel creates hearing and sight in the first forty (per Ḥudhayfa) while the second angel does the same in the third forty (per Ibn Masʿūd on this reading), the two hadiths remain in contradiction.

3 — Abū al-ʿAbbās al-Qurṭubī (656 AH)

Abū al-ʿAbbās al-Qurṭubī — al-Mufhim “The first dhālik (that) is a reference to the place in which the nuṭfa gathered and became a clot, and the second dhālik is a reference to the time, which is the forty.”

The author notes that this gloss — reading “in that place” rather than “in that period of time” — adds nothing intelligible: everyone already knows the embryo is in the womb, whether as nuṭfa, ʿalaqa, or muḍgha. The natural and correct reading is that “in that” means “in that period,” and “the like of that” means the like of that gathering.

4 — Yaḥyā ibn Sharaf al-Nawawī (676 AH)

Al-Nawawī’s position is the fullest articulation of the third-forty view, and is worth setting out at length:

Al-Nawawī — Sharḥ Muslim “The scholars say the way to reconcile these narrations is that the angel is ever-present and watching over the state of the nuṭfa — saying at each stage: ‘O Lord, this is a nuṭfa, this is a clot, this is a morsel of flesh.’ The angel’s speech and actions have specific times. The first is when Allah creates it as a nuṭfa and then moves it to a clot — that is the first time the angel knows it will be a child, for not every nuṭfa becomes a child — and that is after the first forty, at which point it records provision, lifespan, deeds, and whether wretched or blessed. Then the angel has another action at another time, which is its formation and the creation of hearing, sight, skin, flesh, and bone, and whether male or female — and that is only in the third forty, which is the duration of the morsel of flesh, before the close of that forty and before the spirit is breathed in, because the breathing only occurs after the form is complete. As for the saying in one narration ‘when forty-two nights have passed over the nuṭfa, Allah sends to it an angel who forms it and creates its hearing, sight, skin, flesh, and bones, then asks: male or female?’ — al-Qāḍī and others say this is not to be taken at face value; rather the meaning of ‘forming it and creating its hearing…’ is that he records all of that and then does it at another time, because formation right after the first forty is neither found nor usual — it only occurs in the third forty.”

Al-Nawawī’s summary: (1) after the first forty the angel writes provision, lifespan, deeds, and bliss or wretchedness; (2) after the third forty hearing, sight, flesh, bone, and sex are formed, and the spirit breathed; (3) the phrase “forming it and creating its hearing” in Ḥudhayfa’s hadith means recording, not actual creation.

The author’s six rebuttals:

(1) Ḥudhayfa’s hadith and its cognates are explicit that the writing occurs after the first forty; Ibn Masʿūd’s hadith places it after the third forty. Construing the conjunction in Ibn Masʿūd as referring back to the beginning of the sentence (the distant antecedent rather than the near one) is a forced reading, permissible in language but without any contextual or rhetorical motivation here.

(2) The word khalaqa (created/formed) does not mean kataba (wrote/recorded) anywhere in standard Arabic usage — this equation has no lexical precedent.

(3) If recording is in one period and creation in another, that directly conflicts with Ḥudhayfa’s hadith, which places both recording and creation in a single event after one forty.

(4) The medical observation that formation is “not found” after the first forty has been decisively overturned by modern embryology — bone ossification begins precisely at the end of the sixth week.

(5) The scholarly consensus cited (ensoulment after 120 days) is a consensus about ensoulment, not about organ formation — the two are categorically distinct. Consensus is a proof in the absence of texts; it carries no weight when authentic narrations are present.

(6) If thumma (then) were to indicate an 80-day delay, what is the proof for exactly 80 days? It could equally indicate a delay of an hour, or twelve hours.

5 — Abū al-Rabīʿ al-Sarṣarī (716 AH)

Al-Sarṣarī — al-Taʿyīn fī Sharḥ al-Arbaʿīn “The most plausible way to reconcile these two hadiths is to construe them as applying to different embryos — some have the spirit breathed in after 120 days, and some after 42, each hadith specifying a subset of the other.”

The author replies: the wording of the hadith is unrestricted — “the nuṭfa” (al-nuṭfa) and “each of you” (aḥadukum) — there is nothing in either text to confine it to some embryos and not others.

6 — Ibn Rajab al-Ḥanbalī (795 AH)

Ibn Rajab — Jāmiʿ al-ʿUlūm wa-l-Ḥikam “Some have interpreted this as the angel dividing the nuṭfa when it becomes a clot, assigning part to skin, part to flesh, part to bone — planning all of it before its existence. This contradicts the apparent meaning of the hadith; the apparent meaning is that he forms it and creates all these parts… Perhaps this creation consists in forming and dividing it before the flesh and bone exist, and perhaps this applies to some embryos and not others.”

He later adds that some recent commentators held the creation occurs at the start of the second forty, citing Ḥudhayfa’s hadith, and that nothing in Ibn Masʿūd’s hadith refutes this.189

The author’s four rebuttals: (a) khalaqa in the hadith — and in its other wordings (ṣawwaraha, fajaʿalahu) — means bringing into existence, not planning or dividing; this is the dominant usage throughout the Qurʾān and Sunna; (b) the claim that it applies to “some embryos” is negated by the universal wording; (c) the reading of thumma as indicating the order of narration rather than the order of events is an unusual device, and deploying it without a contextual signal misleads the reader without any rhetorical purpose; (d) his analogy with {And He began the creation of man from clay, then made his offspring from an extract of mean water, then He proportioned him and breathed into him of His spirit} (al-Sajda 32:7–9) does not bear the weight placed on it, since the Qurʾānic sentence is not of the same grammatical construction as the hadith.

7 — Ibn Ḥajar al-ʿAsqalānī (852 AH)

Ibn Ḥajar — Fatḥ al-Bārī (summary of al-Qāḍī and Ibn al-Ṣalāḥ) “The reconciliation is to construe the sending of the angel as multiple — once at the start of the second forty, and again at the close of the third for the breathing of the spirit. As for the phrase in Ḥudhayfa’s hadith ‘he forms it,’ the apparent meaning of Ibn Masʿūd’s hadith is that formation only occurs after it becomes a morsel of flesh, so the first is construed as meaning he records and writes the manner of its formation — not that he actually does it — on the grounds that making it male or female only occurs at the morsel stage.”

Then he adds:

Ibn Ḥajar — Fatḥ al-Bārī (his own gloss) “I say: the claim that formation in truth only occurs in the third forty has been challenged, since formation has been observed in many fetuses in the second forty, as has the distinction of male from female. On this basis it may be said that the angel’s first act is to form it in word and writing, then he proceeds to carry it out in deed when the clot is complete — in some embryos this is earlier, in others later.”

The author’s two rebuttals: (a) multiple descents of the angel are an assertion without any chain to support them; (b) “forming it in word and writing” is not what the hadith says — it says khalaqa (created) and ṣawwaraha (formed it), not “wrote its creation.”

8 — Badr al-Dīn al-ʿAynī (855 AH)

Al-ʿAynī — ʿUmdat al-Qārī “I answer that ‘then He sends to it the angel and he is commanded with four words and writes’ is conjoined to ‘he is gathered in his mother’s womb’ and relates to that, not to what precedes it, which is ‘then he becomes a morsel of flesh the like of that’ — and ‘then he becomes a clot’ and ‘then he becomes a morsel’ are parenthetical between the conjunct and what it is conjoined to, and that is permissible and attested in the Qurʾān, the authentic hadith, and classical Arabic.”

This is the distant-antecedent argument, which the author rebuts as above under al-Nawawī.

9 — Ibn al-Mulaqqin (904 AH)

Ibn al-Mulaqqin — al-Tawḍīḥ li-Sharḥ al-Jāmiʿ al-Ṣaḥīḥ “The scholars reconcile these by saying the angel is ever-present… After the first forty it records provision, lifespan, and wretchedness or bliss; then the angel has another action, which is its formation and creating its hearing, sight, and sex — and that is only in the third forty, before the close of that forty and before the spirit is breathed in. The wording ‘when forty-two nights have passed’ is not to be taken at face value, as al-Qāḍī and others said — the meaning of ‘forming it and creating its hearing’ is that he records all of that and then does it at another time.”

The author remarks: this repeats al-Nawawī and al-Qāḍī word for word, so the same rebuttals apply.

10 — Ibn Ḥajar al-Haythamī (974 AH)

Ibn Ḥajar al-Haythamī — al-Fatḥ al-Mubīn “The scholars reconcile these by saying the angel is ever-present… the first time the angel knows it will be a child is when it becomes a clot after the first forty; at that point it writes the four things that follow… Then he says: ‘then I found in a narration from Muslim what refutes the first reconciliation: when forty-two nights have passed, He sends to it an angel who forms it and creates its hearing, sight, flesh, and bone… In this there is explicit statement that the creation of bone is right after the first forty. If we construe creation here as its beginning, and after the third forty as its completion — the first reconciliation is possible; otherwise the second [reconciliation] is necessary.’”

His proposed hidden formation:

Ibn Ḥajar al-Haythamī — al-Fatḥ al-Mubīn (hidden formation) “It may be reconciled by saying that after the first forty the angel is sent for a hidden formation of that clot, then it is sent during the morsel stage or after, as mentioned, and forms it in an apparent formation accompanying the creation of its bone and the like — ponder this, for I have not seen anyone state it explicitly.”

He also offers: perhaps this differs by individual — some are formed after the first forty, others not until the third.190

The author’s rebuttals: the qualifiers “hidden” and “apparent” are not in the hadith text and cannot be inserted without a contextual marker; the hadith says khalaqa and ṣawwaraha — it does not say “a hidden creation.” The unrestricted wording excludes the “some embryos / other embryos” reading.190

He adds two further reconciliation attempts reported in the source:

Ibn Ḥajar al-Haythamī — al-Fatḥ al-Mubīn (dominant-phase reading) “Some have construed Ibn Masʿūd’s hadith as meaning that the embryo is dominated in the first forty by the description of semen (manī), in the second by the description of the clot, and in the third by the description of the morsel — even though its formation has been completed.”

The author replies: this would make “clot” and “morsel” each double — a real one and a dominant-phase one — and this is not in the text.

Ibn Ḥajar al-Haythamī — al-Fatḥ al-Mubīn (two recordings) “Some have reconciled by saying the writing differs by individual: some have it written after the first forty, others after the third — and perhaps this reconciliation is preferable to al-Qāḍī’s.”

The author: the universal language of the hadiths excludes any individual-based qualification.

11 — ʿAbd al-Ḥaqq al-Dihlawī (1052 AH)

ʿAbd al-Ḥaqq al-Dihlawī — Lamaʿāt al-Tanqīḥ “The closest reconciliation is that the first (120-day) hadith is the norm, and the second applies to those born at six months.”

The author: none of the chains of either hadith contain any such qualification; the argument rests on unwarranted assumptions.

12 — Dr. Muḥammad Shāhīn Lāshīn (1423 AH)

Dr. Lāshīn — Fatḥ al-Munʿim “Al-Qāḍī ʿIyāḍ reconciled them by…”

He repeats al-Qāḍī’s argument without addition.

13 — Muḥammad ibn ʿAlī al-Ithyūbī (1426 AH)

Al-Ithyūbī — al-Baḥr al-Muḥīṭ al-Thajjāj “It may be reconciled by saying that it differs by individual embryo.”

The universal language negates this.

Group Two: Those Who Place Organ-Creation at the Start of the Second Forty

These are the scholars who hold that the formation of hearing, sight, flesh, bone, and sex occurs at the end of the first forty and the start of the second (forty-odd nights) — which is the position the scientific evidence in the first half of this study fully confirms.

al-Qāḍī ʿIyāḍ — despite his well-known reconciliation argument above, al-Ithyūbī records that al-Qāḍī also acknowledged a second, simpler reading: that Ibn Masʿūd’s hadith does not specify that the organs form at the end of the third forty — it gives only “the forty” in an unrestricted sense, which is compatible with their forming at the start of the second.191 His interpretation of that as “recording” rather than “creating” is, as the author argues, a weakness, not a strength.

Ibn al-Athīr (630 AH) interprets “gathering” as the nuṭfa’s forty-day settling in the womb in preparation, after which it is created:192

Ibn al-Athīr — al-Nihāya “It may be that ‘gathering’ means the nuṭfa remaining in the womb forty days, fermenting until it is ready for creation and formation, and then it is created after the forty.”

Ibn al-Ṣalāḥ (642 AH) records, through Ibn Ḥajar, that some later commentators inclined to accepting Ḥudhayfa’s hadith at face value — that formation and creation occur in the latter part of the second forty — and that Ibn Masʿūd’s hadith contains nothing to refute this.193

Ibn Taymiyya (728 AH):

Ibn Taymiyya — Majmūʿ al-Fatāwā “The most that can be said about it is that it implies creation may occur in the second forty before it enters the third, and this does not contradict the authentic hadith; nor do we know it to be false. Rather, some women have mentioned that the embryo is created after the forty, and that a male is created before a female. This takes precedence over the jurists who said the embryo cannot be created in less than 81 days, since they based that on the premise that formation only occurs when it has become a morsel, and it cannot become a morsel before 80 days — but formation is possible before that.”

Ibn al-Qayyim (751 AH) gives the most detailed description of embryonic development in the first forty days — the three initial points (heart, brain, liver), the branching blood-lines, the separation of head from shoulder and limbs from ribs, completing at day 40 — citing this as the meaning of “gathering his creation in forty days.”194

Ibn al-Zamlakānī (727 AH) holds that “gathering” means the embryo’s perfection and consolidation, so that by the end of the forty days its form is already sound.195

Ibn Rajab al-Ḥanbalī (795 AH) states explicitly:

Ibn Rajab — Jāmiʿ al-ʿUlūm wa-l-Ḥikam “The apparent meaning of Ibn Masʿūd’s hadith indicates that the formation of the embryo and the creation of its hearing, sight, skin, flesh, and bone is at the start of the second forty — and it follows from this that at the start of the second forty there is already flesh and bone.”

Ibn Ḥajar al-ʿAsqalānī (852 AH) notes in his own gloss (cited above) that the third-forty position “has been challenged, since formation has been observed in many fetuses in the second forty, as has the distinction of male from female,” and that the thumma in Ibn Masʿūd’s hadith may indicate sequence of narration rather than sequence of events.196

Ibn Ḥajar al-Haythamī (974 AH) arrives, after his many reconciliations, at the explicit acknowledgement:

Ibn Ḥajar al-Haythamī — al-Fatḥ al-Mubīn “Then I found in a narration from Muslim [i.e. Muslim 2645] what refutes the first reconciliation… for it contains explicit statement that the creation of bone is right after the first forty.”

ʿAbdullāh ibn Muḥammad al-Ghunaymān (1405 AH):

Al-Ghunaymān — Sharḥ Kitāb al-Tawḥīd “What Ibn Rajab and his teacher Ibn al-Qayyim said is close to what modern physicians determine. Embryos have now become observable through imaging equipment and endoscopes, and among embryologists formation begins early — from within the days of the first forty. The aḥādīth of the Messenger of Allah ﷺ do not contradict reality; the error comes only from misunderstanding what he ﷺ intended.”

Al-Ithyūbī (1426 AH) himself, after all his alternative reconciliations, records Ibn al-Qayyim’s description of embryonic development culminating at day 40, and notes that the organs develop progressively, with hidden formation continuing through the second forty and apparent formation completing at the end of the third.197

Footnotes

  1. [All bones in the body initiate from an ossification center which initially presents as a microscopic locus of bone deposition] — Joe Adserias-Garriga, Age Estimation: A Multidisciplinary Approach, p. 46. Here

  2. [Clavicle is the first bone in the body to ossify. It has membranocartilaginous ossification: most of the bone ossifies in membrane, except its medial end that ossifies in cartilage.] — Rohit Manglik, Human Anatomy with Color Atlas and Clinical Integration, Vol. 1 (Upper Limb) & 2 (Thorax), p. 9 (2024). Here | [the clavicle is the first bone to ossify] — Brian K. Hall, Bones and Cartilage: Developmental and Evolutionary Skeletal Biology, p. 274 (2014). Here

  3. Henry Gray & Peter Llewellyn Williams, Gray’s Anatomy, p. 893. Here

  4. Frederic Shapiro, Pediatric Orthopedic Deformities, p. 10 (2002). Here

  5. Sung-Won Jin, Ki-Bum Sim, Sang-Dae Kim, Development and Growth of the Normal Cranial Vault: An Embryologic Review, p. 193. Here

  6. Grant Breeland, Margaret A. Sinkler, Ritesh G. Menezes, Embryology, Bone Ossification. NIH

  7. Oregon State University, 6.4 Bone Formation and Development. Here

  8. Paper — On Ossification Centers in Human Embryos (Franklin P. Mall). Here

  9. The American Journal of Anatomy, Editorial Board, Charles R. Bardeen et al., p. 442. Here

  10. Ibid, p. 442.

  11. Ibid, p. 456.

  12. Ana Cristina Moreno-Marin et al., Resources for innovative learning of anatomy and foot ossification: Graphic design and virtual reality. Here

  13. Gary C. Schoenwolf, Steven B. Bleyl, Philip R. Brauer, Philippa H. Francis-West, Larsen’s Human Embryology, 6th ed., p. 47.

  14. Satoshi Ogata & Hans K. Uhthoff, The early development and ossification of the human clavicle — an embryologic study. Here

  15. Ibid, p. 330.

  16. Ibid, p. 332.

  17. American Journal of Diseases of Children, Frank Spooner Churchill, L. Emmett Holt, David M. Cowie, John Lovett Morse, Edwin E. Graham, John Howland, Vol. XIV, 1917, pp. 403–405. Here

  18. Francis H. Glorieux, John M. Pettifor, Pediatric Bone, Biology and Diseases, p. 41 (2011). Here

  19. Anne Grethe Jurik, Imaging of the Sternocostoclavicular Region, p. 8 (2007).

  20. Joseph P. Iannotti, Gerald R. Williams, Disorders of the Shoulder, Diagnosis & Management, p. 944. Here

  21. Sir Henry Morris, Human Anatomy, A Complete Systematic Treatise, 1895, p. 119. Here

  22. International Record of Medicine and General Practice Clinics, Vol. 108, p. 308. Here

  23. Owen Arthurs, Post Mortem Imaging of the Fetus & Child, A Comprehensive Guide, p. 23 (2025). Here

  24. Gerard J. Tortora, Bryan H. Derrickson, Principles of Anatomy and Physiology, p. 185 (2020). Here

  25. Sridhar Premkumar, Textbook of Craniofacial Growth, p. 43. Here

  26. Chrystalle Katte Carreon, Dale S. Huff, Eduardo D. Ruchelli, Linda M. Ernst, Color Atlas of Human Fetal and Neonatal Histology, p. 358 (2019). Here

  27. Friedrich Paulsen, Jens Waschke, Sobotta Atlas of Anatomy, Vol. 1, 17th ed., p. 203. Here

  28. Charles Sedgwick Minot, Human Embryology, p. 454. Here

  29. Colorado Medicine, The Journal of the Colorado State Medical Society, Vol. VIII, 1911, p. 108. Here

  30. John Blair Deaver, Upper extremity, back of neck, shoulder, trunk, cranium, scalp, face, p. 266. Here

  31. Leonard Landois, A Manual of Human Physiology, Including Histology and Microscopical Anatomy, Vol. 2, p. 1145. Here

  32. Template: Upper limb ossification table (Mark Hill, UNSW Embryology). Here

  33. Template: Lower limb ossification table (UNSW Embryology). Here

  34. The American Journal of Anatomy, Vol. 5 (1906), p. 453.

  35. Warren C. Hammert, Martin I. Boyer, David J. Bozentka, Ryan Patrick Calfee, ASSH Manual of Hand Surgery, p. 5. Here

  36. V. Subhadra Devi, Textbook of General Anatomy with Systemic Anatomy, Radiological Anatomy, Medical Genetics, p. 69. Here

  37. Henry Gray, Anatomy of the Human Body, Vol. 8, p. 129. Here

  38. Franz Keibel, Manual of Human Embryology, p. 378. Here

  39. Clifford R. Wheeless III, Wheeless’ Textbook of Orthopaedics. Here

  40. D. R. Singh, Essentials of Anatomy for Dentistry Students, p. 111. Here

  41. Warren C. Hammert et al., ASSH Manual of Hand Surgery, p. 5. Here

  42. Vishram Singh, Textbook of Clinical Embryology, 2nd ed., p. 109 (2020). Here

  43. Ronald W. Dudek, Embryology, p. 16. Here

  44. Jones Quain, John Cleland, William Sharpey, Allen Thomson, The Elements of Anatomy, 2nd ed., Vol. 1, pp. 90, 93. Here

  45. Carol A. Chamley, Developmental Anatomy and Physiology of Children, A Practical Approach, p. 74 (2005). Here

  46. Nigel Palastanga, Roger Soames, Anatomy and Human Movement, Structure and Function, p. 11. Here

  47. Rachel Koshi, Cunningham’s Manual of Practical Anatomy, Vol. 1: Upper and Lower Limbs, p. 16. Here

  48. G. Scherer and M. Schmid (eds.), Genes and Mechanisms in Vertebrate Sex Determination, preface, p. 11. Here

  49. Maria I. New, Joe Leigh Simpson, Hormonal and Genetic Basis of Sexual Differentiation Disorders and Hot Topics in Endocrinology: Proceedings of the 2nd World Conference, 2011, p. 37. Here

  50. BGDB Sexual Differentiation — Sex Determination (UNSW Embryology). Here

  51. Jia Jiunn Chew, Genetics of Disorders of Sexual Development, Monash Science Industry Student Placement Program, Prince Henry’s Institute (supervisor: Dr. Rowena Lavery), p. 4. Here

  52. G. Scherer and M. Schmid (eds.), Genes and Mechanisms in Vertebrate Sex Determination, p. 25. Here

  53. BGDB Sexual Differentiation — Sex Determination (UNSW Embryology). Here

  54. Dagmar Wilhelm, Stephen Palmer, Peter Koopman, Sex Determination and Gonadal Development in Mammals, p. 12. Here

  55. Roger V. Short, The Genetics and Biology of Sex Determination, p. 47.

  56. Eric Vilain, Anomalies of Human Sexual Development: Clinical Aspects and Genetic Analysis, UCLA Department of Human Genetics, p. 43.

  57. Ceri Weber, Blanche Capel, Sex Reversal. Here

  58. Michael D. Griswold, Sertoli Cell Biology, p. 61. Here

  59. Mavis Kirkham, Exploring the Dirty Side of Women’s Health, p. 45 (2007). Here

  60. Mandy Wells, Liz Bonner, Effective Management of Bladder and Bowel Problems in Children, p. 48. Here

  61. Stephen S. Wachtel, SRY — Sex Reversal. Here

  62. Ken D. McElreavey, Eric Vilain, Chafika Boucekkine, Michel Vidaud, François Jaubert, François Richaud, Marc Fellous, XY Sex Reversal Associated with a Nonsense Mutation in SRY. Here

  63. Derek J. Chadwick, Jamie A. Goode, The Genetics and Biology of Sex Determination, p. 43. Here

  64. Alberto J. Solari, Sex Chromosomes and Sex Determination in Vertebrates, 2024, p. 226. Here

  65. Yanshe Xie, Changhua Wu, Zicong Li, Zhenfang Wu, Linjun Hong, Early Gonadal Development and Sex Determination in Mammals. Here

  66. Sry: the master switch in mammalian sex determination, Development. Here

  67. Yuria Umemura, Ryosuke Miyamoto, Rie Hashimoto et al., Ontogenic and morphological study of gonadal formation in genetically-modified sex reversal XYPOS mice. Here

  68. Stefanie Kurtz, Schlegelberger et al., Björn Petersen, Knockout of the HMG domain of the porcine SRY gene causes sex reversal in gene-edited pigs (PNAS). Here

  69. Susan Blackburn, Maternal, Fetal, & Neonatal Physiology — E-Book, 2017, p. 20. Here

  70. Anthony W. Norman, Helen L. Henry, Hormones, p. 267. Here

  71. Peter de Boer, The Hidden Relay, How the Germline Connects Generations, p. 134. Here

  72. Germaine Buck Louis, UNEP, ILO, Principles for Evaluating Health Risks in Children Associated with Exposure to Chemicals, 2006, p. 42. Here

  73. Obstetrics & Gynaecology, An Evidence-based Text for MRCOG, 3rd ed., 2016, p. 526. Here

  74. Yisheng Yang, Stephanie Workman, Megan J. Wilson, The molecular pathways underlying early gonadal development, p. 57. Here

  75. Germaine Buck Louis, UNEP, ILO, Principles for Evaluating Health Risks in Children Associated with Exposure to Chemicals, 2006. Here

  76. Kay Elder, Brian Dale, In-Vitro Fertilization, p. 54 (2020). Here

  77. Michael D. Griswold, Sertoli Cell Biology, p. 61. Here

  78. Chrystalle Katte Carreon, Dale S. Huff, Eduardo D. Ruchelli, Linda M. Ernst, Color Atlas of Human Fetal and Neonatal Histology, p. 129. Here

  79. Joydev Mukherji, Rajendra Prasad Ganguly, Subrata Lall Seal, Basics of Gynecology for Examinees, p. 905. Here

  80. David M. Gershenson, Gretchen M. Lentz, Rogerio A. Lobo, Comprehensive Gynecology, 2021, p. 15. Here

  81. Brittany Croft, Thomas Ohnesorg, Andrew Sinclair et al., Human sex reversal is caused by duplication or deletion of core enhancers upstream of SOX9 (Nature Communications). Here

  82. Rosemol Xaviour, Essentials of Human Embryology, 2nd ed. — E-Book, p. 243. Here

  83. Jie Qiao, Peter C. K. Leung, Human Reproductive and Prenatal Genetics, p. 135. Here

  84. Michael K. Skinner, Encyclopedia of Reproduction, p. 409. Here

  85. Dagmar Wilhelm, Stephen Palmer, Peter Koopman, Sex Determination and Gonadal Development in Mammals, p. 12. Here

  86. Singh Rajender, Vutukuri Rajani, Nalini J. Gupta, Baidyanath Chakravarty, Lalji Singh, Kumarasamy Thangaraj, SRY-negative 46,XX male with normal genitals, complete masculinization and infertility. Here

  87. Susan Blackburn, Maternal, Fetal, & Neonatal Physiology — E-Book, 2017, p. 20. Here

  88. Roger V. Short, The Genetics and Biology of Sex Determination, p. 50.

  89. Ceri Weber, Blanche Capel, Sex Reversal. Here

  90. Jia Jiunn Chew, Genetics of Disorders of Sexual Development, Monash / Prince Henry’s Institute, p. 8. Here

  91. Anuja Dokras, Carmen J. Williams, Jerome F. Strauss, Robert L. Barbieri, S. Zev Williams, Yen & Jaffe’s Reproductive Endocrinology — E-Book, p. 161. Here

  92. Defu Ma, Jia-Yi Dong, Li-Qiang Qin, Yuexin Yang, Breast Milk Composition and Infant Metabolism, p. 26. Here

  93. G. Scherer and M. Schmid (eds.), Genes and Mechanisms in Vertebrate Sex Determination, pp. 15, 37. Here

  94. Tommaso Falcone, William W. Hurd, Clinical Reproductive Medicine and Surgery, p. 97. Here

  95. Michael K. Skinner, Encyclopedia of Reproduction, p. 409. Here

  96. G. Scherer and M. Schmid (eds.), Genes and Mechanisms in Vertebrate Sex Determination, p. 14. Here

  97. Roger V. Short, The Genetics and Biology of Sex Determination, p. 26.

  98. Tommaso Falcone, William W. Hurd, Clinical Reproductive Medicine and Surgery, p. 97. Here

  99. Michael K. Skinner, Encyclopedia of Reproduction, p. 409. Here

  100. Jie Qiao, Peter C. K. Leung, Human Reproductive and Prenatal Genetics, p. 135. Here

  101. Jie Qiao, Peter C. K. Leung, Human Reproductive and Prenatal Genetics, p. 128. Here

  102. Genetics of Male Infertility, A Case-Based Guide for Clinicians, 2020, p. 8. Here

  103. Mark A. Sperling, Pediatric Endocrinology, p. 114. Here

  104. Roger V. Short, The Genetics and Biology of Sex Determination, p. 49.

  105. Mark A. Sperling, Pediatric Endocrinology, p. 114. Here

  106. Mark A. Sperling, Pediatric Endocrinology, p. 115. Here

  107. Anuja Dokras et al., Yen & Jaffe’s Reproductive Endocrinology — E-Book, p. 161. Here

  108. Albert Singer et al., The Cervix, p. 7. Here

  109. Richa Saxena, Textbook for MRCOG-1, Basic Sciences in Obstetrics & Gynaecology, 2019, p. 274. Here

  110. Richard A. Polin, William W. Fox, Steven H. Abman, Fetal and Neonatal Physiology, p. 2015. Here

  111. David J. Hurry, David Charles, Obstetrics & Gynecology, 598 Multiple-choice Questions with Referenced, Explanatory Answers, p. 132. Here

  112. Sadhana Gupta, A Comprehensive Textbook of Obstetrics and Gynecology, p. 72. Here

  113. C. R. Kannan, Essential Endocrinology, A Primer for Nonspecialists, 2013, p. 358. Here

  114. Obstetrics & Gynaecology, An Evidence-based Text for MRCOG, 3rd ed., 2016, p. 526. Here

  115. Rodolfo Rey, Nathalie Josso, Chrystèle Racine, Sexual Differentiation, 2020, p. 1. Here

  116. Rodolfo Rey, Nathalie Josso, Chrystèle Racine, Sexual Differentiation, 2020, p. 1. Here

  117. Yanshe Xie, Changhua Wu, Zicong Li, Zhenfang Wu, Linjun Hong, Early Gonadal Development and Sex Determination in Mammals. Here

  118. Yisheng Yang, Stephanie Workman, Megan J. Wilson, The molecular pathways underlying early gonadal development, p. 57. Here

  119. Elizabeth Kostas-Polston, Heather S. Hubbard, Ivy M. Alexander, Joyce Cappiello, Versie Johnson-Mallard, Women’s Healthcare in Advanced Practice Nursing, p. 131. Here

  120. Angela K. Lucas-Herald, Rod T. Mitchell, Testicular Sertoli Cell Hormones in Differences in Sex Development. Here

  121. Mavis Kirkham, Exploring the Dirty Side of Women’s Health, p. 45 (2007). Here

  122. Mandy Wells, Liz Bonner, Effective Management of Bladder and Bowel Problems in Children, p. 48 (2007). Here

  123. Chrystalle Katte Carreon, Dale S. Huff, Eduardo D. Ruchelli, Linda M. Ernst, Color Atlas of Human Fetal and Neonatal Histology, p. 129. Here

  124. Scott F. Gilbert, Developmental Biology, 6th ed. (2000), Primary and Secondary Sex Determination. Here

  125. Richard J. Martin, Avroy A. Fanaroff, Michele C. Walsh, Fanaroff and Martin’s Neonatal-Perinatal Medicine — E-Book, 2019, p. 1666. Here

  126. Jie Qiao, Peter C. K. Leung, Human Reproductive and Prenatal Genetics, p. 135. Here

  127. Albert Altchek, Liane Deligdisch, Nathan Kase, Diagnosis and Management of Ovarian Disorders, p. 3. Here

  128. Shixuan Wang, Ovarian Aging, p. 9. Here

  129. AWHONN, Core Curriculum for Neonatal Intensive Care Nursing — E-Book, 2020, p. 556. Here

  130. Anna Maria Fulghesu, Good Practice in Pediatric and Adolescent Gynecology, 2017, p. 37. Here

  131. Louis R. Kavoussi, Campbell-Walsh-Wein Urology — E-Book, 2020, p. 995. Here

  132. Louis R. Kavoussi, Campbell-Walsh-Wein Urology — E-Book, 2020, p. 993. Here

  133. Louis R. Kavoussi, Campbell-Walsh-Wein Urology — E-Book, 2020, p. 994. Here

  134. Maria I. New, Oksana Lekarev, Alan Aspar­as, Tony T. Yuen, Bert O’Malley, Gary D. Hammer (eds.), Genetic Steroid Disorders, 2013, p. 88. Here

  135. Diagnostic Gynecologic and Obstetric Pathology, p. 5. Here

  136. Jia Jiunn Chew, Genetics of Disorders of Sexual Development, Monash / Prince Henry’s Institute, p. 4. Here

  137. G. Scherer and M. Schmid (eds.), Genes and Mechanisms in Vertebrate Sex Determination, p. 17. Here

  138. Richard J. Martin, Avroy A. Fanaroff, Michele C. Walsh, Fanaroff and Martin’s Neonatal-Perinatal Medicine — E-Book, 2019, p. 1667. Here

  139. Roger V. Short, The Genetics and Biology of Sex Determination, p. 46.

  140. Al-Sunna by Abū Bakr ibn al-Khallāl (3/539).

  141. Al-Ṭabarānī, al-Muʿjam al-Ṣaghīr, no. 455. Here

  142. Al-Sunna by Abū Bakr Aḥmad ibn Muḥammad ibn Hārūn al-Khallāl (d. 311 AH), ed. Dr. ʿAṭiyya al-Zahrānī, Dār al-Rāya, vol. 1, p. 540.

  143. Al-Ṭabarānī, al-Muʿjam al-Kabīr (9/233).

  144. al-Qadar by Ibn Wahb (p. 69, ed. al-Ḥifyān). 2

  145. Ibn Ḥajar, Fatḥ al-Bārī (11/479, Salafiyya ed.). 2

  146. Ṣaḥīḥ al-Bukhārī (8/122).

  147. Ṣaḥīḥ Muslim (8/44).

  148. al-Qadar by Ibn Wahb (ed. al-ʿUthaym), p. 151.

  149. Sharḥ Mushkil al-Āthār (9/485).

  150. Juzʾ min ḥadīth Abī ʿAlī al-Ṣawwāf, p. 47 (Shāmila auto-numbering).

  151. al-Qadar by al-Firyābī, p. 101.

  152. Muʿjam Shuyūkh Ibn al-Aʿrābī (2/502, Ibn al-Jawzī ed.).

  153. Aḥādīth Abī al-Ḥusayn al-Kilābī, p. 65 (Shāmila auto-numbering).

  154. Min ḥadīth Sufyān al-Thawrī (ed. ʿĀmir Ṣabrī), p. 159.

  155. Musnad Ibn al-Jaʿd, p. 379.

  156. Mustakhraj Abī ʿAwāna (20/191).

  157. Mustakhraj Abī ʿAwāna (20/193).

  158. Mishyakha Ibn Ṭahmān, p. 139.

  159. al-Qadar by Ibn Wahb (ed. al-ʿUthaym), p. 153.

  160. Musnad Abī Dāwūd al-Ṭayālisī (1/238).

  161. Muṣannaf ʿAbd al-Razzāq (10/189, 2nd Taʾṣīl ed.).

  162. al-Jāmiʿ of Maʿmar ibn Rāshid (11/123). 2

  163. Musnad al-Ḥumaydī (1/221).

  164. Musnad Aḥmad (6/125, Risāla ed.). 2

  165. Musnad Aḥmad (7/48, Risāla ed.).

  166. Musnad Aḥmad (7/169, Risāla ed.).

  167. Ṣaḥīḥ al-Bukhārī (4/111).

  168. Ṣaḥīḥ al-Bukhārī (4/133). 2

  169. al-Sunna by Ibn Abī ʿĀṣim (1/77).

  170. al-Sunan al-Kubrā by al-Nasāʾī (Risāla ed., 10/130).

  171. al-Sunna by Abū Bakr ibn al-Khallāl (3/538).

  172. al-Musnad by al-Shāshī (2/140).

  173. Sunan Ibn Mājah (1/54, ed. al-Arnaʾūṭ).

  174. Musnad Aḥmad (6/13, Risāla ed.).

  175. al-Sunna by Abū Bakr ibn al-Khallāl (3/539).

  176. al-Muʿjam al-Kabīr by al-Ṭabarānī (9/233).

  177. Ṣaḥīḥ Muslim (8/45).

  178. Ṣaḥīḥ Muslim (8/46).

  179. al-Sunna by Ibn Abī ʿĀṣim (1/79).

  180. al-Sunna by Ibn Abī ʿĀṣim (1/80).

  181. al-Adab al-Mufrad (ed. ʿAbd al-Bāqī), p. 107. 2

  182. al-Ibāna al-Kubrā by Ibn Baṭṭa (4/226). 2

  183. al-Ibāna al-Kubrā by Ibn Baṭṭa (4/26). 2

  184. al-Qadar by Ibn Wahb (p. 67, ed. al-Ḥifyān). 2

  185. al-Qadar by al-Firyābī, p. 111. 2

  186. al-Fatḥ al-Mubīn bi-Sharḥ al-Arbaʿīn by Ibn Ḥajar al-Haythamī, p. 205; Fatḥ al-Qawī al-Matīn by ʿAbd al-Muḥsin al-ʿAbbād, p. 37; al-Baḥr al-Muḥīṭ al-Thajjāj by al-Ithyūbī (41/313).

  187. Al-ʿAynī, ʿUmdat al-Qārī (3/293).

  188. Fatḥ al-Bārī by Ibn Ḥajar (11/485, Salafiyya ed.).

  189. Jāmiʿ al-ʿUlūm wa-l-Ḥikam (p. 145, ed. al-Faḥl).

  190. al-Fatḥ al-Mubīn, p. 203. 2

  191. al-Baḥr al-Muḥīṭ al-Thajjāj (41/311).

  192. al-Nihāya fī Gharīb al-Ḥadīth wa-l-Athar (1/297).

  193. Fatḥ al-Bārī by Ibn Ḥajar (11/485, Salafiyya ed.).

  194. Sharḥ Kitāb al-Tawḥīd min Ṣaḥīḥ al-Bukhārī by al-Ghunaymān (2/214).

  195. al-Burhān al-Kāshif ʿan Iʿjāz al-Qurʾān (cited in multiple sources with reference to p. 275).

  196. Mashāriq al-Anwār al-Wahhāja (2/428).

  197. al-Baḥr al-Muḥīṭ al-Thajjāj (41/313).

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